Identification of the novel recessive gene pi55(t) conferring resistance to Magnaporthe oryzae  

作　　者：HE XiuYing LIU XinQiong WANG Li WANGLing LIN Fei CHENG YongSheng CHEN ZhaoMing LIAO YaoPing PAN QingHua 

机构地区：[1]State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources,Laboratory of Plant Resistance and Genetics,College of Natural Resources and Environment Sciences,South China Agricultural University,Guangzhou 510642,China [2]Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China [3]Key Biotechnology Laboratory of State Ethnic,Affairs Commission,College of Life Science,South-Central University for Nationalities,Wuhan 43074,China

出　　处：《Science China(Life Sciences)》2012年第2期141-149,共9页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant No. U1131003);the National Transgenic Research Projects(Grant Nos. 2009ZX08009-023 and 2011ZX08001-002);the Guangdong Provincial Natural Science Foundation (Grant No. 10151064001000008);the President Science Foundation of Guangdong Academy of Agricultural Sciences (Grant No. 201101)

摘　　要：The elite rice cultivar Yuejingsimiao 2 （YJ2） is characterized by a high level of grain quality and yield, and resistance against Magnaporthe oryzae. YJ2 showed 100% resistance to four fungal populations collected from Guangdong, Sichuan, Liaoning, and Heilongjiang Provinces, which is a higher frequency than that shown by the well-known resistance （R） gene donor culti- vats such as Sanhuangzhan 2 and 28zhan. Segregation analysis for resistance with F2 and F4 populations indicated the re- sistance of YJ2 was controlled by multiple genes that are dominant or recessive. The putative R genes of YJ2 were roughly tagged by SSR markers, located on chromosomes 2, 6, 8, and 12, in a hulked-segregant analysis using genome-wide selected SSR markers with F4 lines that segregated into 3 resistant （R）： 1 susceptible （S） or 1R：3S. The recessive R gene on chromosome 8 was further mapped to an interval ~1.9 cM/152 kb in length by linkage analysis with genomic position-ready markers in the mapping population derived from an F4 line that segregated into 1R：3S. Given that no major R gene was mapped to this inter- val, the novel R gene was designated as pi55（t）. Out of 26 candidate genes predicted in the region based on the reference genomic sequence of the cultivar Nipponbare, two genes that encode a leucine-rich repeat-containing protein and heavy-metalassociated domain-containing protein, respectively, were suggested as the most likely candidates for pi55（t）.The elite rice cultivar Yuejingsimiao 2 (YJ2) is characterized by a high level of grain quality and yield, and resistance against Magnaporthe oryzae. YJ2 showed 100% resistance to four fungal populations collected from Guangdong, Sichuan, Liaoning, and Heilongjiang Provinces, which is a higher frequency than that shown by the well-known resistance (R) gene donor cultivars such as Sanhuangzhan 2 and 28zhan. Segregation analysis for resistance with F 2 and F 4 populations indicated the resistance of YJ2 was controlled by multiple genes that are dominant or recessive. The putative R genes of YJ2 were roughly tagged by SSR markers, located on chromosomes 2, 6, 8, and 12, in a bulked-segregant analysis using genome-wide selected SSR markers with F 4 lines that segregated into 3 resistant (R):1 susceptible (S) or 1R:3S. The recessive R gene on chromosome 8 was further mapped to an interval 1.9 cM/152 kb in length by linkage analysis with genomic position-ready markers in the mapping population derived from an F 4 line that segregated into 1R:3S. Given that no major R gene was mapped to this interval, the novel R gene was designated as pi55(t). Out of 26 candidate genes predicted in the region based on the reference genomic sequence of the cultivar Nipponbare, two genes that encode a leucine-rich repeat-containing protein and heavy-metalassociated domain-containing protein, respectively, were suggested as the most likely candidates for pi55(t).

关 键 词：rice blast recessive resistance gene pi55 resistance inheritance gene mapping 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] S511.03[农业科学—植物保护]