油菜硫甙性状QTL区间的加密和整合  

Fine Mapping and Integration of Glucosinolate QTL in Brassica Napus

作　　者：曾丽萍[1] 冯吉[1] 龙艳[1] 孟金陵[1] 

机构地区：[1]华中农业大学植物科学技术学院,湖北武汉430070

出　　处：《现代生物医学进展》2012年第1期52-55,共4页Progress in Modern Biomedicine

摘　　要：目的:加密油菜控制硫甙性状QTL区间,并进行QTL整合预测候选基因。方法:利用生物信息学方法根据已知测序的白菜BAC序列信息设计引物,在油菜TN DH群体中进行多态性扩增和定位,并根据加密后构建的遗传连锁图重新检测QTL,进行QTL整合。结果:将根据白菜BAC设计的3对多态性标记成功定位到油菜控制硫甙性状QTL区间,进行QTL整合后将QTL置信区间进一步缩小,并判定了初步的候选基因。结论:充分利用白菜已测序的BAC或者基因组信息,将能加快油菜基础研究的步伐。Objective： Adding markers to the confidence interval of glucosinolate content QTL in Brassica napus,and then to do QTL integration and candidate gene prediction.Methods： Designed primers according to BAC sequence of Brassica rapa,and then detected polymorphism between the two parents of TN DH population of B.napus.With the polymorphic markers genetic mapping will be done in TN DH population.Finally,QTL mapping and integregation will be done based on the new linkage map.Results： Three polymorphic markers were mapped to the confidence interval of seed glucosinolate content QTL,after QTL mapping and integregation were done,the confidence interval was narrow down and two candidate genes were predicted in the region.Conclusion： If taking full advantage of the sequenced BAC and whole genomic information,the basic research of B.napus will be speeded up.

关 键 词：油菜 硫甙 QTL 

分 类 号：Q341[生物学—遗传学] Q37