急性日本血吸虫病与血清白介素-33的关系研究  

Correlation between acute schistosomiasis japonica and serum interleukin-33 levels

作　　者：陈若青[1] 周艺彪[1] 蒋露芳[1] 宋秀霞[1] 张志杰[1] 居丽雯[1] 姜庆五[1] 

机构地区：[1]复旦大学公共卫生学院流行病学教研室,公共卫生安全教育部重点实验室,上海200032

出　　处：《中国血吸虫病防治杂志》2012年第1期32-34,39,共4页Chinese Journal of Schistosomiasis Control

基　　金：国家高技术研究发展计划(863计划)(2006AA02Z402)

摘　　要：目的检测急性日本血吸虫病患者外周血血清白介素-33（IL-33）水平,探讨IL-33在急性日本血吸虫病疾病进展中的作用。方法选择来自湖区的4例急性日本血吸虫病患者和来自非疫区的15例对照者作为研究对象,收集人口学资料并采集肘静脉血。采用酶联免疫吸附试验检测所有研究对象的外周血血清IL-33水平。应用Stata10.0软件进行数据的统计学分析。结果急性日本血吸虫病患者血清IL-33水平为517.33（334.65,1056.88）pg/ml,显著高于对照者血清IL-33水平1.66（1.66,6.35）pg/m（lZ=-3.207,P=0.001）。急性日本血吸虫病患者血清IL-33水平与外周血嗜酸粒细胞计数、患者感染时长的相关系数均为0.8,相关系数均无统计学意义（P=0.2）。结论 IL-33在急性日本血吸虫病患者血清中显著升高,在日本血吸虫病急性期发挥了促进炎症作用,并可能在患者感染日本血吸虫后7~9周参与启动Th2型免疫应答。Objective To study the function of interleukin-33 （IL-33） in the development of human acute schistosomiasis japonica by determining the serum IL-33 levels in acute schistosomiasis japonica patients. Methods Four patients with acute schistosomiasis japonica were recruited from schistosomiasis endemic lake areas, and 15 controls were recruited outside the schistosomiasis endemic areas. The demographic data and venous blood were collected from all the subjects. The serum IL-33 levels of all the subjects were tested by using enzyme-linked immunosorbent assay. All the results were statistically analyzed with Stata 10.0 software. Results The serum IL-33 levels of the patients with acute schistosomiasis japonica [517.33 （334.65, 1 056.88） pg/ml] were significantly higher than those of the controls [1.66（1.66, 6.35） pg/ml] （Z= -3.207, P = 0.001）. The correlation coefficients between serum IL-33 levels and numbers of eosinophils, serum IL-33 levels and duration of infection were both 0.8 （P = 0.2）. Conclusions The serum IL-33 level is significantly elevated in the patients with acute schistosomiasis japonica, indicating that IL-33 may play a pro-inflammatory role in the acute stage of schistosomiasis japonica and participate in initiating the Th2 type immune responses between 7 and 9 weeks after the infection.

关 键 词：急性日本血吸虫病 白介素-33 Th1/Th2型免疫应答 

分 类 号：R532.21[医药卫生—内科学]