基于油镜的全自动显微图像扫描采集系统改进和优化及在疟疾血片复审的应用  

Improvement of fully automatically microscopic scanning system based on the oil-lens and its application in reading of malaria blood smears

作　　者：高原[1] 纵兆民[1] 杨佩才[1] 张洪英[1] 魏德会[1] 李燕青[1] 黄景章[2] 周晓农[3] 

机构地区：[1]江苏省南京市疾病预防控制中心,南京210003 [2]江苏省南京市卫生局 [3]中国疾病预防控制中心寄生虫病预防控制所

出　　处：《中国血吸虫病防治杂志》2012年第1期67-71,共5页Chinese Journal of Schistosomiasis Control

基　　金：江苏省卫生厅项目(X200925)

摘　　要：目的对基于油镜的全自动显微图像扫描采集系统(图象系统)软件部分进行修改和优化,并观察该改进和优化后的系统在疟疾血片复审中的应用效果。方法对基于油镜的全自动显微图像扫描采集系统进行了软件部分的修改和优化。用改进和优化后的图像系统对云南省腾冲县的4个乡(镇)医疗机构门诊镜检站镜检员人工常规镜下读片的115张疟疾血片进行复审,比较该图像系统和人工镜下读片的准确度、敏感度和特异度。结果改进和优化后的图像系统扫描速度得到了提升,实际操作更加方便简捷。扫描的图片自动保存后,可以反复研读,集体判读。并可以通过网络建立血片复审机制和远程诊断。在疟疾血片复审中显示,图像系统诊断疟疾的整体准确度为100%,高于人工常规镜检的准确度(95.5%)(P=0.002);敏感度和特异度均为100%,亦高于人工常规镜检法(分别为91.30%和94.52%)(P均<0.05)。结论改进和优化后的图象系统功能强大,扫描速度快,操作简便快捷,在疟疾读片的速度、准确度、敏感度和特异度方面均优于人工常规镜下读片,适于在基层推广使用。Objective To improve the automatically microscopic scanning system based on the oil-lens and apply it in reading of malaria blood smears. Methods The software of the system was modified and optimized. A total of 115 blood films from Tengchong City of Yunnan Province were examined by the automatically systematic method or read by blood smear examination staff of Tengchong City and the accuracy, sensitivity and specificity were determined and compared between the two methods. Results The scanning of the system was speedier and the actual operation was more simple and convenient. The accuracy, sensitivity and specificity of the system were better than those of the professional staff. There was a significant difference of accuracy between the two methods （P = 0.002） and there was a significant difference of comprehensive sensitivity and specificity between the two methods （P 0.01）. Moreover, the system had the function of automatically saving scanning pictures which could be read again by everyone through the Internet. Conclusions The fully automatically microscopic scanning system based on the oil-lens has many functions and the operation is simple. It can improve the speed, accuracy, sensitivity and specificity of malaria blood smears and those indices are much better than those of the reading smears under a microscope by the professional staff. Therefore, it is easier to be applied at the grassroots level.

关 键 词：疟原虫 油镜 全自动 扫描 镜检 复审 

分 类 号：R531.3[医药卫生—内科学]