高糖膳食对载脂蛋白AI基因-75 G/A多态性不同基因型健康青年血脂及载脂蛋白比值的影响  

Effects of a High-carbohydrate Diet on the Serum Lipid and Apolipoprotein Ratios in Healthy Young Adults with Different Genotypes of APOA1-75 G/A Polymorphism

作　　者：宋永燕[1,2] 欧国进[1] 龚仁蓉[3] 张珍[1] 胡敏珊[1] 樊梅[1] 李元昊[1] 方定志[1] 

机构地区：[1]四川大学华西基础医学与法院学院生物化学与分子生物学教研室,成都610041 [2]川北医学院基础医学院生物化学教研室,四川南充637000 [3]四川大学华西医院胸心外科,成都610041

出　　处：《中国医学科学院学报》2012年第1期1-7,共7页Acta Academiae Medicinae Sinicae

基　　金：教育部新世纪优秀人才支持计划(NCET-04-0863)~~

摘　　要：目的探讨高糖膳食对载脂蛋白AI基因(APOA1)启动子区-75 G/A多态性不同基因型健康青年血脂及载脂蛋白比值的影响。方法给予56名平均年龄为(22.89±1.80)岁的健康青年7 d平衡膳食和6 d高糖膳食。平衡膳食的热量组成为15%蛋白质、31%脂肪和54%碳水化合物;高糖膳食的热量组成为15%蛋白质、15%脂肪和70%碳水化合物。于膳食干预的第1天、第8天和第14天清晨抽取12 h空腹静脉血,测定血脂及载脂蛋白浓度,计算甘油三酯(TG)/高密度脂蛋白胆固醇(HDL-C)、总胆固醇(TC)/HDL-C、低密度脂蛋白胆固醇(LDL-C)/HDL-C及载脂蛋白B100(APOB100)/载脂蛋白AI(APOAI)的比值。提取全基因组DNA,聚合酶链式反应-限制性片段长度多态性法分析APOA1-75 G/A多态性。结果基础值时,血脂及载脂蛋白比值在男性基因型之间的差异无统计学意义(P>0.05);在女性中,A等位基因携带者LDL-C/HDL-C显著高于GG基因型受试者(P<0.05)。高糖膳食后,TC/HDL-C在各性别和基因型分组中均显著降低(P<0.01)。LDL-C/HDL-C在男性各基因型中显著降低(P<0.05);在女性中,LDL-C/HDL-C仅在A等位基因携带者中显著降低(P<0.01),在GG基因型受试者中差异无统计学意义(P>0.05)。结论 APOA1-75 G/A多态性A等位基因可能对其女性携带者的LDL-C/HDL-C比值有一定的影响。To investigate the effects of a highcarbohydrate diet on the lipid and apoli poprotein ratios in healthy young adults with different genotypes of the polymorphism at 75 site in the promoter region of the gene of apolipoprotein AI （ APOA1 ）. Methods Fiftysix subjects aged （22. 89 ± 1.80） years were given a washout diet for 7 days, followed by a highcarbohydrate diet for 6 days. The washout diet contained 15% protein, 31% fat, and 54% carbohydrate. The highcarbohydrate diet contained 15 % protein, 15% fat, and 70% carbohydrate. Twelvehour fasting serum lipids and apolipoproteins B100 and AI were measured on the mornings of the 1st, the 8th, and the ldth days from the beginning of the washout diet. The ratios of triglyceride （TG）/high-density lipoprotein cholesterol （ HDLC ）, total cholesterol （ TC ）/HDLC, lowdensity lipoprotein cholesterol （LDLC）/HDLC, and apolipoprotein B100 （APOB100）/apolipoprotein AI （APOAI） were calculated. The genome DNA was extracted and the polymorphism of APOA1 75 G/A was determined by polymerase chain reaction followed by restriction fragment length polymorphism assay. Results At baseline, the lipid and apolipoprotein ratios showed no significant differences between the GG genotype and the A carriers in males （ P 〉 0.05 ）, whereas the female A carriers had a significantly higher ratio of LDLC/ HDLC compared with the female subjects with the GG genotype （ P 〈 0 diet, significant decreases of TC/HDLC were found in all the groups, 05 ）. Following the highcarbohydrate regardless of sex and genotype （ P 〈 0.01 ）. LDLC/HDLC experienced significant decreases in both the genotypes in males （ P 〈 0.05 ） , while in females, significant decrease of LDLC/HDLC was only observed in A carriers （ P 〈 0.01 ）. Conclusion The A allele of the 75 G/A polymorphism in APOA1 may have specific effects on the LDLC/HDLC ratio in females.

关 键 词：载脂蛋白AI 基因多态性 高糖膳食 血脂及载脂蛋白比值 

分 类 号：R713.8[医药卫生—妇产科学]