26例有偿献血员HIV-1基因分型与变异特征  

Genotyping and variability of HIV-1 in 26 cases of paid blood donors

作　　者：郭飞飞[1] 陈国敏[1] 曾毅[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京100052

出　　处：《中华实验和临床病毒学杂志》2012年第1期37-39,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家“973”项目（NO.2005CB529903和2006CB504207）资助

摘　　要：目的分析某地区有偿献血人员中流行的人免疫缺陷病毒1型（HIV-1）gag、pol、env基因亚型及基因变异特征。方法提取HIV-1感染者外周血单核细胞（PBMC）DNA，经巢式PCR（NestedPCR）扩增gag（p17-p24）、pol（PR＊RT）、env（C2-V5）基因片段，纯化测序后用MEGA5．0等生物学软件对核苷酸序列进行分析。结果23份样本为B亚型，2份为B亚型与C亚型重组，1份为CRF01-AE与B亚型重组。PR区未发现蛋白酶抑制剂主要耐药性突变，RT区检测到核苷类逆转录酶抑制剂耐药性突变M184V和非核苷类逆转录酶抑制剂耐药性突变K101E，G190A。结论流行于该地区的HIV-1毒株以B亚型为主。大多数毒株对常规抗病毒药物仍然敏感，使用HARRT治疗方案依然有效。CXCR4型辅助受体的毒株顶端四肽多为GPGR（91．7％），提示GPGR可能与疾病的进展有关。Objective To analyze the genome mutations of HIV-1 gag, pol and env genes from HIV-infected paid blood donors in rural central China. Methods DNA was extracted from peripheral blood mononuclear ceils, gag（p17-p24）,pol（PR-RT）,env（C2-V5） genes were amplified by nested polymerase chain reaction （PCR） , purified products were sequenced, and sequence data was analyzed by MEGA5.0 soft wares. Results Twenty-three samples were subtype B, two samples were recombinant of subtype B and subtype C, one sample was recombinant of subtype CRF01_AE and subtype B. PI major resistance mutations were not found in the PR region. M184V, KIOIE and G190A were detected in the RT region, respectively. Conclusion Subtype B was the major HIV circulating genetic forms in this area. Most strains were sensitive to high active anti-retroviral therapy （ HARRT）. 91.7% V3 loop tip motifs of X4-tropic strains was GPGR. It showed that GPGR might be associated with accelerate disease progression to AIDS.

关 键 词：HIV-1 基因型 种系发生 受体 HIV 

分 类 号：R512.91[医药卫生—内科学]