外源性前列腺素E2对兔跟腱胶原含量的影响  

EFFECTS OF EXOGENOUS PROSTAGLANDIN E2 ON COLLAGEN CONTENT OF Achilles TENDON OF RABBITS IN VIVO

作　　者：李辉[1] 唐康来[1] 邓银栓[1] 谢美明[1] 常德海[1] 陶旭[1] 许建中[1] 

机构地区：[1]第三军医大学西南医院骨科全军矫形外科中心,重庆400038

出　　处：《中国修复重建外科杂志》2012年第3期352-358,共7页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金面上项目(30872620;81071464);重庆市自然科学基金重点项目(CSTC2011BA5010);全军医学科研"十一五"计划面上项目(06MB245)~~

摘　　要：目的肌腱损伤后周围前列腺素E2(prostaglandin E2,PGE2)及体外牵伸载荷条件下肌腱细胞产生的PGE2均升高,通过观察外源性PGE2对兔跟腱胶原含量的影响,探讨其与肌腱病发生的关系。方法取健康3～4月龄日本短耳兔24只,体重2.0～2.5 kg,雌雄不限;根据PGE2注射剂量不同随机分为两组(n=12):低剂量组(50 ng)和高剂量组(500 ng)。随机选择一侧后肢跟腱中部经皮注射0.2 mL对应剂量PGE2,对侧对应部位注射0.2 mL生理盐水作为对照,每周注射1次至处死。注射4、8周后两组各处死6只实验动物,大体观察跟腱情况后,取双侧跟腱HE染色观察细胞结构、基质形态,苦味酸-天狼星红染色偏振光显微镜下观察肌腱组织的胶原变化并定量分析Ⅰ、Ⅲ型胶原含量,透射电镜观测胶原纤维密度及直径。结果 HE染色示两组实验侧均出现胶原纤维结构破坏。苦味酸-天狼星红染色示4、8周时两组实验侧Ⅲ型胶原纤维均较对照侧增加,Ⅰ型胶原减少(P<0.05);与低剂量组比较,高剂量组实验侧Ⅲ型胶原纤维增加,Ⅰ型胶原减少,Ⅲ/Ⅰ型胶原比值增高(P<0.05)。透射电镜示两组4、8周时实验侧单位面积内总胶原纤维横截面积所占百分比均较对照侧降低,直径>100 nm的纤维比例均较对照侧降低,直径<100 nm的纤维比例均增加(P<0.05);与低剂量组比较,高剂量组实验侧单位面积内总胶原纤维横截面积所占百分比更低,直径>100 nm的纤维比例均明显降低,直径<100 nm的比例明显增加(P<0.05)。结论重复注射PGE2能导致兔跟腱Ⅰ型胶原减少,Ⅲ型胶原增多,Ⅰ/Ⅲ型胶原比例倒置,单位面积总胶原纤维密度降低,直径变细,可能与肌腱病发生相关。Objective Prostaglandin E2（PGE2） production increases in human tendon fibroblasts after the tendon injuries and repetitive mechanical loading in vitro.To analyze the relations between PGE2 and tendinopathy by observing the changes of collagen content and proportion after the Achilles tendon of rabbits is repeatedly exposed to PGE2.Methods Twenty-four Japanese rabbits（aged 3-4 months,weighing 2.0-2.5 kg,and male or female） were equally randomized into 2 groups according to injection dose of PGE2： low dose group（50 ng） and high dose group（500 ng）.Corresponding PGE2（0.2 mL） was injected into the middle segment of the Achilles tendon of hindlimb,the same dose saline into the same site of the other side as controls once a week for 4 weeks or 8 weeks.The Achilles tendons were harvested at 4 and 8 weeks after injection.HE staining was used to observe the cell structure and matrix,and picric acid-sirius red staining to observe the distribution and types of collagen fibers,and transmission electron microscopy was used to measure the density of the unit area and diameter of collagen fibers.Results HE staining showed that collagen structural damage was observed in low dose and high dose groups.Picric acid-sirius red staining showed that the content of type I collagen significantly decreased while the content of type III collagen significantly increased in experimental side of 2 groups at 4 and 8 weeks after injection when compared with control sides（P 0.05）.The content of type I collagen was significantly lower and the content of type III collagen and ratio of type III to type I were significantly higher in high dose group than in low dose group（P 0.05）.Transmission electron microscopy showed that the collagen fibers density of unit area was significantly lower and the diameter was significantly smaller in high dose and low dose groups than in the controls（P 0.05）,and in high dose group than in low dose group（P 0.05）.Conclusion Repeat exposure of the Achilles tendon of rabbit to PGE2 ca

关 键 词：前列腺素E2 肌腱病 胶原 兔 

分 类 号：R686[医药卫生—骨科学]