地锦草洗脱部位指纹图谱特征与其抗真菌作用的灰关联度分析  

Grey relational analysis on fingerprint characteristics of different eluted parts of Euphorbia humifusa and antifungal effect

作　　者：李治建 周露 古力娜.达吾提 斯拉甫.艾白 

机构地区：[1]新疆维吾尔自治区维吾尔医药研究所,新疆乌鲁木齐830049 [2]新疆维吾尔医方剂学实验室,新疆乌鲁木齐830049 [3]新疆维吾尔自治区维吾尔医医院,新疆乌鲁木齐830049

出　　处：《中国中药杂志》2012年第5期580-584,共5页China Journal of Chinese Materia Medica

基　　金：新疆维吾尔自治区自然科学基金项目(2011211B52);国家自然科学基金项目(81060370;81160557)

摘　　要：目的:确定地锦草不同洗脱部位指纹图谱特征峰所代表的化学成分对其抗真菌作用贡献大小,为建立"指纹图谱与药效关联"的中药质量控制模式提供理论依据和数据支持。方法:用HPLC研究地锦草不同洗脱部位的指纹图谱;用NC-CLS M38-A研究其抗真菌作用;用灰关联度分析方法研究其谱效关系。结果:地锦草不同洗脱部位的抗真菌作用是多种化学成分共同作用的结果,各特征峰所代表的各化学成分(以特征峰编号作为该特征峰所代表的化学成分名称)对其抗真菌作用贡献的大小顺序为红色毛癣菌:2>5>3>4>1;石膏样毛癣菌:5>2>3>4>1。结论:地锦草不同洗脱部位指纹图谱与其抗真菌作用之间有一定的对应关系。Objective： To determine the contribution of chemical components represented by characteristic peaks of fingerprint of different eluted parts of Euphorbia humifusa to the antifungal effect to provide theoretical basis and data support for the traditional Chinese medicine quality control mode.Method： HPLC was introduced to analyze fingerprint of different eluted parts of E.humifusa.The antifungal effect was observed using NCCLS M38-A method.The correlation between fingerprint and antifungal effect of E.humifusa was identified using grey relational analysis.Result： The antifungal effect of different eluted parts of E.humifusa is resulted from the combined action of multiple chemical components.Chemical components representing a contribution to pharmacy dynamics were ordered by representative spectrum 25341 for anti-Trichophyton rubrum and anti-T.mentagrophytes 52341.Conclusion： Fingerprint of different eluted parts of E.humifusa is related with their antifungal effect to some extent.

关 键 词：地锦草 指纹图谱 抗真菌 灰关联度 谱效关系 

分 类 号：R284[医药卫生—中药学]