基于中药血清化学及血清药理学方法探讨香青兰保护心肌细胞缺氧/复氧损伤物质基础  

Study on material basis of Dracocephalum moldavica for protecting cardiomyocyte against hypoxia/reoxygenation injury by traditional Chinese medicine serum chemical and pharmacological methods

作　　者：田友清[1,2] 尚靖[1] 何婷[1] 蔡旻煊[1] 阿布卡德[1] 

机构地区：[1]中国药科大学新药筛选中心,江苏南京210009 [2]江苏联合职业技术学院连云港中医药分院,江苏连云港222006

出　　处：《中国中药杂志》2012年第5期620-624,共5页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2009ZX09401-007);中国药科大学中央高校基本科研业务费专项资金研究生项目(JKY2009025)

摘　　要：目的:利用中药血清化学及血清药理学方法探讨香青兰保护心肌细胞缺氧/复氧损伤的物质基础。方法:测定和比较香青兰提取物及其灌胃给药前后大鼠空白血清和含药血清的HPLC,寻找香青兰入血成分;体外培养乳大鼠原代心肌细胞及H9c2细胞,用Na2S2O4或N2为缺氧剂建立缺氧/复氧损伤模型,以细胞活力、LDH释放量、T-SOD活力、MDA产量及细胞凋亡为指标,考察香青兰提取物及其含药血清、香青兰提取物不同给药剂量及不同给药时间段所采血清的处理物对细胞损伤的影响。结果:香青兰在血清中出现4个移行成分,其中3个为原型成分,1个可能为代谢产物;与模型组或空白血清组相比,香青兰提取物、含药血清及不同因素影响下的血清处理物显著降低缺氧/复氧心肌细胞LDH释放量和MDA产量(P<0.05,P<0.01),显著升高T-SOD及细胞活力(P<0.05,P<0.01),明显抑制细胞凋亡。结论:香青兰提取物4个入血成分可能为香青兰保护心肌细胞缺氧/复氧损伤的物质基础。Objective： To study the material basis of Dracocephalum moldavica for protecting cardiomyocyte against hypoxia/reoxygenation injury by using traditional Chinese medicine（TCM） serum chemical and pharmacological methods.Method： The extract of D.moldavica（DME） and its content absorbed into blood were determined,while blank serum and medicinal serum of rats before and after intragastrical administration of DME were also compared by HPLC.The Na2S2O4 or N2-based hypoxia/reoxygenation injury model was established by cultivating primary neonate rat cardiomyocytes or H9c2 cells in vitro.Cell viability,LDH release,T-SOD activity,MDA production and apoptosis were detected to learn the effect of DME,medicated serum and different treatments of medicinal serums under different dosage and action duration of DME on cardiomyocyte against hypoxia/reoxygenation injury.Result： Four transitional components of DME absorbed into blood after intragastrical administration were found,three of which were original components and one possible metabolite.Furthermore,compared with the model group or the blank serum group,LDH release and MDA production（P0.05,P0.01） of DME extracts,medicated serum or different treatments of medicinal serum under different dosage and action duration of DME.However,T-SOD and cell viability were improved significantly（P0.05,P0.01）,while apoptosis of cardiomyocytes were also obviously inhibited.Conclusion： The four components absorbed into blood are probably the material basis of DME used for protecting cardiomyocyte agastin hypoxia/reoxygenation injury.

关 键 词：香青兰 血清药物化学 血清药理学 缺氧/复氧损伤 原代心肌细胞 H9c2细胞 

分 类 号：R285.5[医药卫生—中药学]