灯盏乙素淀粉微球经离体鼻黏膜的渗透性评价  

Scutellarin starch microspheres′ permeability through nasal mucosa of different animals in vitro

作　　者：叶赏和[1] 宋晓玲[2,3] 李昌煜[3] 葛卫红[3] 石森林[3] 

机构地区：[1]金华市人民医院,浙江金华321000 [2]包头医学院药学院,内蒙古包头014060 [3]浙江中医药大学药学院,浙江杭州310053

出　　处：《中国中药杂志》2012年第5期663-666,共4页China Journal of Chinese Materia Medica

基　　金：国家教育部重点项目(211072);浙江省实验动物科技计划项目(2009F80016)

摘　　要：目的:研究灯盏乙素淀粉微球经动物离体鼻黏膜的渗透性。方法:采用立式扩散池,分别以新鲜的离体犬、小型猪、家猪鼻黏膜为渗透屏障,灯盏乙素淀粉微球(以灯盏乙素计0.25 mg)置于鼻黏膜上方,以空白pH 6.8 PBS为接收液,进行渗透试验(n=4),HPLC测定灯盏乙素。结果:灯盏乙素淀粉微球经离体犬、小型猪、家猪鼻黏膜渗透系数分别为(5.295±0.637)×10-2,(4.065±1.140)×10-2,(1.855±0.150)×10-2m.L-1;灯盏乙素淀粉微球不同动物离体鼻黏膜渗透系数大小顺序为犬>小型猪>家猪;灯盏乙素淀粉微球经犬与家猪离体鼻黏膜的渗透系数、经小型猪与家猪离体鼻黏膜的渗透系数之间存在显著性差异。结论:灯盏乙素淀粉微球中药物可经上述离体鼻黏膜渗透;渗透系数可能存在种属之间的差异。Objective： To study scutellarin starch microspheres′ permeability through nasal mucosa of different animals in vitro.Method： The Franz diffusion cell method was used to experiment the permeability test（n=4）,taking fresh nasal mucosa of dog,swine and domestica in vitro as permeation barrier separately,with scutellarin starch microspheres（scutellarin 0.25 mg） above them,and blank pH6.8 PBS as absorption liquid to detemine the scutellarin by HPLC.Result： The permeability coefficient of scutellarin starch microspheres through nasal mucosa of dog,swine and domestica in vitro were（5.295±0.637）×10-3,（4.065±1.140）×10-3,（1.855±0.150）×10-3 cm·mL-1 separately.The permeability coefficient order of scutellarin starch microspheres through nasal mucosa of different animals in vitro is dog swine domestica,and there are significant differences between the permeability coefficient of scutellarin starch microspheres through nasal mucosa of dog,swine in vitro,and that through nasal mucosa of swine and domestica in vitro.Conclusion： Drugs in scutellarin starch microspheres could permeate through the above-mentioned nasal mucosa in vitro.There might be different permeability coefficient among different species.

关 键 词：灯盏乙素 淀粉微球 鼻黏膜 渗透系数 

分 类 号：R285.5[医药卫生—中药学]