溶质在两种反相色谱柱中的动力学色谱行为  

Dynamic chromatographic behavior of solutes on two reverse phase columns

作　　者：李建军[1] 贺娜[1] 王骊丽[1] 

机构地区：[1]西北大学合成与天然功能分子化学教育部重点实验室,西安710069

出　　处：《分析试验室》2012年第3期66-69,共4页Chinese Journal of Analysis Laboratory

基　　金：陕西省自然科学基金项目(2006B28)资助

摘　　要：研究了不同流速下4种溶质在两种不同反相色谱柱上的色谱图及它们的动力学色谱性质,进一步验证了溶质相对保留值(RRT)与流动相流速之间存在良好的双对数线性关系,并对溶质在流速改变时洗脱顺序发生改变的现象从动力学因素上进行了合理的解释;同时还发现色谱填料的孔径及拓扑学构型不同将导致溶质在其上具有不同的动力学色谱参数及动力学色谱行为。Different chromatograms of four solutes were obtained by reversed-phase liquid chromatography （RPLC） with two chromatographic columns at different flow rates. The dynamic chromatographic behavior of solutes in RPLC was studied and the results confirmed that the logarithm of the relative retention time （RRT） of solutes was proportional to the logarithm of the flow rate of mobile phase （v）. The reason for that the elution order changed with change of the flow rate of mobile phase was explained reasonable with dynamic factors of solutes. We also found that the solutes would have different dynamic chromatographic parameters and behaviors if the pore-size and topology configuration of chromatographic packings were not the same.

关 键 词：流速 液相色谱 洗脱顺序 保留值 

分 类 号：O657.7[理学—分析化学]