化妆品中2种限用呫吨染料的高效液相色谱检测  

Determination of two xanthene dyes in cosmetics by high performance liquid chromatography

作　　者：马艳凤[1] 李琼[1] 武晓剑[1] 崔俭杰[1] 

机构地区：[1]上海应用技术学院上海香料研究所,上海200232

出　　处：《分析试验室》2012年第3期70-72,共3页Chinese Journal of Analysis Laboratory

基　　金：国家质量监督检验检疫总局公益性行业科研专项项目(2009424267)资助

摘　　要：建立了化妆品种中2种限用呫吨染料酸性黄73和溶剂橙16的高效液相色谱/二极管阵列检测器检测方法。在ODS C18反相色谱柱上,流动相为0.1 mol/L乙酸铵-甲醇-乙腈(体积比为65:17.5:17.5)等度洗脱,检测波长为492 nm,柱温35℃,流速1.0 mL/min。以保留时间定性,外标法定量。在5～500μg/mL范围内,酸性黄73和溶剂橙16的质量浓度与各自相应的峰面积呈现良好的线性关系,仪器检出限均为0.5μg/mL。样品前处理方法采用甲醇作溶剂超声提取,酸性黄73和溶剂橙16的加标回收率分别在91.2%～106.2%和93.2%～101.5%范围内。Acid yellow 73 and solvent orange 16, two determined simultaneously by high performance liquid restricted synthetic xanthene dyes used in cosmetics, were chromatography with diode array detector. The method was performed on an ODS Cls reversed-phases column using isocratic elution with a mobile phase composed of 65% 0. 1 mol/L aqueous ammonium acetate, 17.5% methanol and 17.5% acetonitrile. The detection wavelength was 492 nm, and the column temperature was 35 ℃ while the flow rate of the mobile phase was 1.0 mL/min. Under the chromatographic conditions established, acid yellow 73 and solvent orange 16 were analyzed qualitatively based on their retention times and quantitatively by external standard method. The calibration curves showed good linearity between 5μg/mL and 500μg/mL for acid yellow 73 and solvent orange 16, respectively. And the limits of detection were both 0. 5 μg/mL. The pretreatment of cosmetic samples was carried out by ultrasonic extraction using methanol as extract solvent and the average recoveries of analytes spiked in cosmetics from 91.2% to106.2% for acid yellow 73 and from 93.2% to 101.5% for solvent orange 16.

关 键 词：化妆品 呫吨染料 高效液相色谱 检测 

分 类 号：O657.7[理学—分析化学]