硫普罗宁的药代动力学及光度法测定  

Study on pharmacokinetics and spectrophotometric determination of tiopronin

作　　者：乔月纯[1,2] 占海红[3] 李全民[1] 

机构地区：[1]河南师范大学化学与环境科学学院,新乡453007 [2]河南机电高等专科学校电气工程系,新乡453002 [3]平顶山教育学院生化系,平顶山467000

出　　处：《分析试验室》2012年第3期83-85,共3页Chinese Journal of Analysis Laboratory

基　　金：河南省科技攻关计划(092102310340);河南省基础与前沿技术研究计划(102300410255)项目资助

摘　　要：建立了光度法测定硫普罗宁的新方法。研究表明:在SCN-和KNO3存在下,控制溶液pH4.0,Cu(II)被硫普罗宁还原生成的Cu(I)与SCN-反应形成CuSCN沉淀,该沉淀能浮在水相表面。通过测定溶液中剩余Cu(II)的量,可以测定硫普罗宁的含量。吸光度与硫普罗宁浓度之间存在良好线性关系。线性方程:A=4.898-0.3616ρ(μg/mL),线性范围为0.25～12.0μg/mL,相关系数R=0.9993,检出限为0.16μg/mL。该方法可直接用于药物中硫普罗宁含量的测定及其药代动力学行为研究。A novel spectrophotometric method has been developed to determine tiopronin in this paper. The study indicated that in the presence of SCNand KNO3, Cu （Ⅱ ） was reduced to Cu （ Ⅰ） by the thiol group （-SH） of tiopronin and the resulting Cu （ Ⅰ ） was precipitated with SCN-to form the CuSCN precipitation that could be suspended in the upper part of water phase at pH 4. 0. By determining the residual amount of copper （ Ⅱ）, the indirect determination of tiopronin can be achieved. Beer＇ s Law is obeyed in the range of 0. 25× 12. 0 μg/mL of tiopronin at 510 nm with a linear correlation coefficient of 0. 9993. The detection limit （LOD） （3 cr/k） is 0. 16 mg/L. The method was successfully applied to the determination of tiopronin in pharmaceutical preparations and its pharmacokinetics.

关 键 词：分光光度法 Cu(Ⅱ) 硫普罗宁 药代动力学 

分 类 号：O657.3[理学—分析化学]