牛精清及牛用稀释液在猪精液冷冻中的应用  

Application of bull seminal plasma and bull semen special extender to boar semen cryopreservation

作　　者：胡传活[1,2] 王献伟[1,2] 胡传水[3] 杨俊涛[1,2] 凌泽继[2] 卢晟盛[1,2] 韦英明[2] 卢克焕[1,2] 

机构地区：[1]广西大学动物繁殖研究所,广西南宁530005 [2]广西大学动物科技学院,广西南宁530005 [3]广西农业职业技术学院,广西南宁530007

出　　处：《广东农业科学》2012年第3期119-121,共3页Guangdong Agricultural Sciences

基　　金：广西大学科研基金(XBZ091008);广西教育厅科研项目(201012MS001)

摘　　要：为了验证牛精清及牛用精液冷冻稀释液在猪精液冷冻中的有效性,将牛精清(0、2.50%、5.00%、10.00%)加入猪精液冷冻稀释液(葡萄糖1.10%、柠檬酸1.48%、Tris 2.42%、NAC 0.02%、卵黄20.00%、甘油6.00%、青霉素0.06%、链霉素0.10%)中,或用改良猪精液冷冻稀释液(另加入0.10 mmol/L他克林)、牛精液冷冻稀释液及改良牛精液冷冻稀释液,对猪精液进行冷冻处理。结果表明,在本试验条件下,牛精清对猪精子冷冻保存没有正面作用;改良牛用稀释液用于猪的精液冷冻,效果与改良猪用稀释液相当,优于未改良牛用稀释液。The objective of the present study was to validate the effect of bull seminal plasma or bull semen special extender for freezing on boar semen cryopreservation.Bull seminal plasma(0,2.50%,5.00% and 10.00%) were added to the boar semen diluents(glucose1.10%,citric acid 1.48%,Tris 2.42%,NAC 0.02%,egg yolk 20.00%,glycerol 6.00%,penicillin 0.06%,streptomycin 0.10%) or modified boar semen special extender for freezing(containing 0.10 mmol/L tacrine),bull semen special extender for freezing and modified bull semen special extender for freezing(containing 0.10 mmol/L tacrine) were used.The results showed that:(1) under the conditions of this experiment no improvement on the quality of frozen-thawed boar spermatozoa was observed while bull seminal plasma was supplemented in diluents;(2)The quality of spermatozoa frozen in modified bull semen diluents was similar to those frozen in modified boar semen diluents(both supplemented with tacrine),and superior to those frozen in unmodified bull semen diluents(without tacrine).

关 键 词：猪精液冷冻保存 牛精清 牛用精液冷冻稀释液 

分 类 号：S814.3[农业科学—畜牧学]