大鼠PTSD后蓝斑核β-catenin的表达下降伴随细胞凋亡  

Expression of β-catenin in locus coeruleus neurons of rats with posttraumatic stress disorder

作　　者：闫胜男[1] 韩芳[1] 石玉秀[1] 

机构地区：[1]中国医科大学基础医学院组织学与胚胎学教研室病理与病理生理学研究所,沈阳110001

出　　处：《中国组织化学与细胞化学杂志》2012年第1期1-4,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金(30600341);教育部博士点基金(20092104110016)

摘　　要：目的探讨创伤后应激障碍(PTSD)大鼠蓝斑神经元β-catenin(β-连环蛋白)的表达变化。方法采用国际认定的SPS方法刺激建立大鼠PTSD模型,取成年健康雄性Wistar大鼠100只,随机分为连续单一刺激(single prolongedstress,SPS)模型1d、4d、7d、14d组和对照组,应用免疫组化、免疫印迹方法检测PTSD大鼠蓝斑神经元β-catenin的表达变化;透射电镜观察PTSD大鼠蓝斑神经元的超微结构变化。结果经SPS刺激后大鼠蓝斑神经元细胞内β-catenin于1d开始逐渐减少,14d表达最少;蓝斑神经元出现细胞凋亡改变。结论蓝斑神经元细胞凋亡可能是导致PTSD患者蓝斑功能失调的重要原因之一。Objective To investigate the expression of β-catenin in the locus coeruleus of rats with post-traumatic stress disorder （PTSD） . Methods The SPS-method was used to set up the rat PTSD models. A total of 100 health , male Wistar rats were randomly divided into 1, 4, 7 and 14d groups of single prolonged stress （ SPS ） and a normal control group. Immunohistochemistry , immunofluorescence,and western blot were used to observe and detect the changes in the expression of β-catenin in the locus coeruleus neurons, while ultrastructure was detected by electron microscopy. Results Apoptotic cells were present in the locus coeruleus of PTSD rats. The expression of β-catenin in the locus coeruleus gradually decreased from ld after SPS, and reached the minimum at SPS 14d. Conclusion The changes of β-catenin expression in the locus coeruleus may play important roles in the dysfunction of the locus coeruleus in PTSD patients

关 键 词：创伤后应激障碍 蓝斑 Β-CATENIN 细胞凋亡 大鼠 

分 类 号：R338.26[医药卫生—人体生理学]