NGAL对大鼠肾脏缺血再灌注损伤时肾小管上皮细胞凋亡的保护作用  

Protective effect of NGAL on renal tubular epithelial cell apoptosis in rat renal ischemia/reperfusion injury

作　　者：宫丽[1] 于华[2] 诸葛一峰 于青[1] 

机构地区：[1]上海交通大学附属第一人民医院肾内科,上海200080 [2]青岛大学附属心血管病医院

出　　处：《临床肾脏病杂志》2012年第2期85-88,共4页Journal Of Clinical Nephrology

基　　金：基金项目：上海市浦江人才计划项目基金课题（030432）

摘　　要：目的探讨中性粒细胞明胶酶相关脂质运载蛋白（neutrophil gelatinase associated lipocalin，NGAL）对大鼠肾脏缺血再灌注损伤肾小管上皮细胞凋亡及凋亡蛋白fas，bcl-2表达的影响。方法建立大鼠肾脏缺血再灌注损伤模型，用HE染色观察肾组织病理变化情况；TUNEL法检测肾小管上皮细胞凋亡；免疫组织化学方法检测fas，bcl-2蛋白表达，并利用图像分析系统测量阳性表达率进行定量分析。结果①缺血再灌注模型组肾小管上皮细胞凋亡数为（20．8±3．7）个／高倍视野，NGAL组为（8．6±3．4）个／高倍视野，2组差异有统计学意义（P〈0．05）。②NGAL组较缺血再灌注模型组fas阳性表达率下降，差异有统计学意义（P〈0．05）；bcl-2阳性表达率增强，差异有统计学意义（P〈0．05）。结论NGAL对大鼠缺血再灌注损伤的肾小管上皮细胞有保护作用，其作用可能与减少细胞凋亡、改变凋亡蛋白的表达有关系。Objective To investigate the effects of NGAL on renal tubular epithelial cell apopto sis and apoptosis-associated protein fas bcl-2 in rat renal ischemia/reperfusion injury. Methods Renal ischemia/reperfusion injury models of rats were established, the pathological changes of renal tissue were observed by hemotoxylin-eosin （HE） staining, renal tubular epithelial cell apoptosis was detected by TUNEL method, the expression of fas and bel-2 proteins was measured by immunohistochemical staining, and the positive rate of protein expression was quantitatively examined by imaging analysis system. Results （1） The number of apoptotic cells was （20. 8 ± 3. 7）per field in ischemic/reperfusion group, and（8. 6 ± 3. 4）per field in NGAL group（P〈0. 05） ;（2）As compared with ischemic/reperfusion group, the positive rate of fas expression was increased（P〈0. 05）, while that of bcl-2 expression decreased（P〈0. 05）in NGAL group. Conclusions NGAL can protect injured renal tubular epithelial cells probably by decreasing cell apoptosis and regulating the protein expression by apoptosis-regulating cytokines.

关 键 词：大鼠 再灌注损伤 细胞凋亡 凋亡调节蛋白质类 

分 类 号：R699.2[医药卫生—泌尿科学]