棕榈酸致大鼠L6肌细胞胰岛素抵抗与JNK1活化的关系  

Association of JNK1 activation with palmitic acid-induced insulin resistance in L6 rat muscle cells

作　　者：杨宁宁[1] 王佑民[1] 陈冬[1] 陈明卫[2] 

机构地区：[1]安徽医科大学内分泌与代谢病研究所,合肥230022 [2]安徽医科大学第一附属医院内分泌科,合肥230022

出　　处：《安徽医科大学学报》2012年第3期241-244,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽高校省级自然科学研究重点项目(编号:KJ2011A161)

摘　　要：目的建立棕榈酸(PA)诱导的大鼠L6肌细胞胰岛素抵抗模型,初步探讨其机制与C-jun氨基末端激酶1(JNK1)活化的关系。方法不同浓度PA分别培养已分化的L6肌细胞2、4、8、12、24、36 h,用葡萄糖试剂盒分别检测各组培养液中剩余葡萄糖浓度,观察不同浓度PA对骨骼肌细胞葡萄糖摄取的影响。MTT法检测棕榈酸对L6细胞活性的影响。建立L6肌细胞胰岛素抵抗模型,Western blot检测正常组(NG组)和胰岛素抵抗组(IR组)的JNK1和p-JNK1的蛋白表达水平。结果 0.4 mmol/L的PA孵育24～36 h与0.6、0.8 mmol/L的PA孵育8～24 h后细胞上清液葡萄糖浓度明显高于NG组(P<0.05),MTT结果显示0.8、1.0 mmol/L的PA作用24、36 h时对细胞的活性有影响。Western blot结果显示:NG组JNK1和p-JNK1的表达量较低,IR组JNK1和p-JNK1的表达量上升,其中p-JNK1表达量上升较JNK1明显,但与NG组比较,IR组的JNK1表达差异无统计学意义,p-JNK1、p-JNK1/JNK1表达差异均有统计学意义(P<0.05)。结论通过一定浓度和时间的PA刺激可导致大鼠L6成肌细胞胰岛素抵抗的形成,其机制可能和JNK1的活化有一定关系。Objective To establish palmitic acid（PA） induced insulin resistance（IR） model in rat L6 muscle cells,and initially explore its mechanism and the association with the activation of JNK1.Methods Rat L6 skeletal muscle cells was cultured by different concentration of PA for 2,4,8,12,24 and 36 h respectively.The concentration of remained glucose in each group was measured by Glucose Test Kit respectively,and observed the effects of different concentration of PA on glucose uptake in L6 skeletal muscle cells.The effects of PA to L6 skeletal muscle cells activity were measured by MTT.Protein expression of JNK1 and p-JNK1 in normal group and in IR group with PA-induced were measured by Western blot.Results When incubated in 0.4 mmol/L PA for 12~36 h,or in 0.6 and 0.8 mmol/L PA for 8~24 h,the concentration of glucose remained in PA group was significantly higher than that in normal group（P0.05）.MTT results suggested that incubated in 0.8,1.0 mmol/L PA for 24~36 h had effected on cells activity.The protein expression of JNK1 and p-JNK1 in NG group was low,while it was higher in IR group,especially for p-JNK1.But compared with normal group,the expression of JNK1 in IR group had no statistical significance,the expression of p-JNK1 in IR group was significantly higher（P0.05）.Conclusion A certain concentration of PA may lead to IR in rat L6 muscle cells,which may be associated with JNK1 activation.Inhibition of excessive activation of JNK1 may be a new target for treatment of IR.

关 键 词：L6肌细胞 胰岛素抵抗 JNK 棕榈酸 

分 类 号：R587.1[医药卫生—内分泌] R589.2[医药卫生—内科学]