人脐带间充质干细胞对肺气肿模型大鼠细胞因子的影响  

Effect of human umbilical cord mesenchymal stem cells on cytokines in rat model of emphysema

作　　者：程雪松[1] 王叶芳[1] 王锦[1] 韩晓燕[1] 汪伟民[1] 

机构地区：[1]安徽医科大学第一附属医院老年呼吸内科,合肥230022

出　　处：《安徽医科大学学报》2012年第3期282-285,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅自然科学基金资助项目(编号:KJ2009A118)

摘　　要：目的研究外源性人脐带间充质干细胞(HUMSC)对肺气肿大鼠模型中细胞因子水平的影响。方法建立猪胰蛋白酶诱导的肺气肿模型,40只雄性SD大鼠随机分为HUMSC组(经尾静脉输注1×106个HUMSC/ml)、PBS组(经尾静脉输注PBS 1 ml)、模型组(不予任何干预)、假手术组,每组10只。干预2周后处死大鼠,ELISA法检测血清及肺泡灌洗液(BALF)中白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)及白细胞介素10(IL-10)的表达水平。结果与假手术组比较:HUMSC、PBS、模型组血清及BALF中IL-1β、TNF-α水平显著升高(P<0.05),IL-10水平显著降低(P<0.05);给予HUMSC干预后血清TNF-α及BALF中IL-1β、TNF-α水平较模型组、PBS组明显下降(P<0.05),而血清IL-1β未见明显改变;血清及BALF中IL-10水平均明显升高(P<0.05)。结论 HUMSC干预可以影响肺气肿大鼠血清及BALF中IL-1β、TNF-α及IL-10的表达,对肺气肿大鼠炎症反应有调节作用,可能是干预肺气肿形成、修复肺组织的机制之一。Objective To investigate the effect of exogenous adminstration of human umbilical cord mesenchymal stem cells（HUMSC） on cytokine levels in rat model of the porcine pancreatic elastase（PPE） induced emphysema.Methods Forty male adult rats were randomly divided into 4 groups： emphysema＋HUMSC-treated group,emphysema＋PBS-treated group,emphysema group and sham-operated group（n=10）.Emphysema＋HUMSC-treated group was treated with 1×106 HUMSC cultured in 1.0 ml PBS by tail vein.Emphysema＋PBS-treated group was treated with 1.0 ml phosphate-buffered saline（PBS） by tail vein.Emphysema group and sham-operated group were untreated.All rats were sacrificed at day 14 post-treatment.The expression levels of IL-1β,TNF-α and IL-10 were detected in serum and bronchoalveolar lavage fluid（BALF） by ELISA.Results Compared with sham-operated group,the levels of IL-1β,TNF-α in BALF and serum were all increased in emphysema group,emphysema＋HUMSC transplantation group,emphysema＋PBS transplantation group,but there were statistical significance only in BALF level of TNF-α,serum levels of IL-1β and TNF-α.The BALF and serum levels of IL-10 in BALF and serum was significantly increased.Conclusion The transplantation of HUMSC have modulator effects on main cytokines of emphysema rats in vivo.HUMSC can downregulate the IL-1β,TNF-α expression and upregulate the IL-10 expression,which may be play a potential role on intervention of emphysema and lung tissue repair.

关 键 词：肺气肿 人脐带间充质干细胞 白细胞介素1Β 肿瘤坏死因子Α 白细胞介素10 

分 类 号：R563.3[医药卫生—呼吸系统] R392.4[医药卫生—内科学]