Antagonistic Effect of Russula virescens(Schaeff.) Fr.against Bortrytis cinerea  

绿菇(Russula virescens(Schaeff.)Fr.)对灰霉菌(Bortrytis cinerea)的拮抗作用研究(英文)

作　　者：黄小琴[1] 刘勇[1] 张蕾[1] 周西全[1] 

机构地区：[1]四川省农业科学院植物保护研究所,四川成都610066

出　　处：《Agricultural Science & Technology》2012年第3期669-671,681,共4页农业科学与技术（英文版）

基　　金：Supported by Applied Foundation Project of Science and Technology Department of Sichuan Province (2008JY0021-2)~~

摘　　要：[Objective] The paper was to study the antagonistic effect of Russula virescens （Schaeff.） Ft. against Bortrytis cinerea. [Method] Using the confrontation culture method, the antagonistic effect of mycelium, fermentation broth and mycelial extract of R. virescens against B. cinerea was studied. [Result] The antagonistic ef- fect of R. vivesscens against B. cinerea was mainly manifested as hyperparasitism of mycelium and inhibition effect of metabolites. The inhibition rate of confrontation growth was between 46.9% and 52.3%, and hyperparasitism phenomenon was obvi- ous. The fermentation broth of R. virescens cultured by PDB medium had the strongest growth inhibition effect, and the growth inhibition rate against B. cinerea was the highest of 61.59%. The thermal stability of fermentation broth was good, the mycelial extract of R. virescens had no inhibition effect against the growth of B. cinerea. [Conclusion] The study provided theoretical basis for subsequent differentiation and drug sensitivity of B. cinerea.[目的]研究绿菇（Russula virescens（Schaeff．）Fr．）对灰霉菌的拮抗作用。[方法]采用对峙培养测定法研究了绿菇菌丝体，发酵液及菌丝体提取液对灰霉菌的拈抗效果。[结果]绿菇对灰霉菌的拈抗作用主要表现为菌丝体重寄生和代谢产物生长抑制作用，对峙生长抑制率介于46．9％～52．3％，重寄生现象明显。PDB培养基培养绿菇获得的发酵液生长抑制作用最强，对辣椒灰霉菌生长抑制率最高，为61．59％；发酵液热稳定性良好；绿菇菌丝提取液对供试灰霉菌无生长抑制作用。[结论]该研究为灰霉菌的分化和药敏感性研究提供了理论依据。

关 键 词：Russula virescens（Schaeff.）Fr. Bortrytis cinerea Antagonistic effect 

分 类 号：S436.46[农业科学—农业昆虫与害虫防治]