枸骨叶的化学成分研究  

Chemical constituents from leaves of Ilex cornuta

作　　者：周思祥[1] 姚志容[1] 李军[1] 屠鹏飞[1] 

机构地区：[1]北京大学药学院天然药物及仿生药物国家重点实验室,北京100191

出　　处：《中草药》2012年第3期444-447,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30672608);教育部长江学者与创新团队基金项目(985-2-063-112)

摘　　要：目的研究枸骨Ilex cornuta叶的化学成分。方法利用硅胶、凝胶等柱色谱和半制备高效液相色谱对枸骨叶的化学成分进行分离纯化,利用光谱数据结合理化性质鉴定其结构。结果分离得到了12个化合物,分别鉴定为(2R,3S,4S)-4-(4-hydroxy-3-methoxybenzyl)-2-(5-hydroxy-3-methoxyphenyl)-3-hydroxymethyl-tetrahydrofuran-3-ol(1)、大黄素甲醚(2)、11-酮基-α-香树脂棕榈酸酯(3)、羽扇豆醇(4)、30-醛基羽扇豆醇(5)、乌索醇(6)、3β-羟基-乌索-11-烯-13β(28)-内酯(7)、乌索酸(8)、β-谷甾醇(9)、胡萝卜苷(10)、坡模酸-28-O-β-D-葡萄糖苷(11)、长梗冬青苷(12)。结论化合物1和2为首次从冬青属中分离得到,化合物11和12为首次从该植物中分离得到。Objective To study the chemical constituents in the leaves of Ilex cornuta.Methods The compounds were isolated and purified by silica gel,Sephadex LH-20 column chromatography,and semi-preparative HPLC,and their structures were identified by spectroscopic data and physicochemical properties.Results Twelve compounds were obtained from the leaves of I.cornuta and identified as（2R,3S,4S）-4-（4-hydroxy-3-methoxybenzyl）-2-（5-hydroxy-3-methoxyphenyl）-3-hydroxymethyl-tetrahydrofuran-3-ol（1）,physcion（2）,11-keto-α-amyrin palmitate（3）,lupole（4）,30-oxolupeol（5）,uvaol（6）,3β-hydroxy-urs-11-en-13β（28）-olide（7）,ursolic acid（8）,β-sitosterol（9）,daucosterol（10）,pomolic acid-28-O-β-D-glucopyranoside（11）,and pedunculoside（12）.Conclusion Compounds 1 and 2 are obtained from the plants in Ilex L.for the first time,and compounds 11 and 12 are obtained from this plant for the first time.

关 键 词：枸骨叶 冬青属 大黄素甲醚 坡模酸-28-O-β-D-葡萄糖苷 长梗冬青苷 

分 类 号：R284.1[医药卫生—中药学]