亚临界水提取葛根中总异黄酮的研究  

Study on total isoflavone from Puerariae Radix by subcritical water extraction

作　　者：周丽[1] 张博雅[1] 张永忠[1,2] 

机构地区：[1]东北农业大学理学院应用化学系,黑龙江哈尔滨150030 [2]教育部大豆生物学重点实验室,黑龙江哈尔滨150030

出　　处：《中草药》2012年第3期492-495,共4页Chinese Traditional and Herbal Drugs

基　　金：国家"863"计划课题(2008AA10Z331)

摘　　要：目的考察亚临界水提取葛根总异黄酮的最佳提取工艺。方法通过单因素试验和正交试验,以总异黄酮提取率为指标,优选出葛根总异黄酮的提取工艺参数。结果当提取温度为120℃,提取时间为30 min,料液比为1∶25,压力为1.1 MPa时,葛根总异黄酮的提取率和质量分数分别为7.83%、24.9%。结论与传统的提取方法相比,亚临界水提取技术是新发展起来的一种新型提取技术,具有提取时间短、效率高、环境友好等优点。Objective To optimize the extracting condition of total isoflavone from Puerariae Radix by subcritical water extraction.Methods Taking isoflavones yield as index,the technology parameter of subcritical water extraction of Puerariae Radix total isoflavone was optimized by single factor test and orthogonal test.Results The optimum extraction parameters were as follows： temperature 120 ℃,extraction time 30 min,solid-liquid ratio 1∶25,pressure 1.1 MPa.The yield of Puerariae Radix total isoflavone in the extracts was 7.83%,and the purity of the isoflavone was 24.9%.Conclusion Compared with traditional extracting methods,subcritical water extraction is a new developing technology in recent years.Its advantage is fast,effective,and environmental friendly.

关 键 词：亚临界水提取技术 葛根 总异黄酮 单因素试验 工艺优化 

分 类 号：R284.2[医药卫生—中药学] R286.02[医药卫生—中医学]