密度对黑腹果蝇胚细胞系L-2/M delta 2-3冻存效果的影响  

Cryopreservation of Cell Line L-2/M delta 2-3 from Embryo of Drosophila melanogaster:The Effects of Cell Density on the Cytoactive

作　　者：丁伟峰[1] 冯颖[1] 张欣[1] 李娴[1] 

机构地区：[1]中国林业科学研究院资源昆虫研究所国家林业局资源昆虫培育与利用重点实验室,云南昆明650224

出　　处：《林业科学研究》2012年第1期6-10,共5页Forest Research

基　　金：林业公益性行业科研专项(4-38)

摘　　要：使用双翅目昆虫黑腹果蝇(Drosophila melanogaster Fallen)胚细胞系L-2/M delta 2-3作为研究材料,制备10个密度试验组(0.81×1062.88×107个.mL-1,每组跨度大于2.00×106个.mL-1),分别在冻后第6、10、14个月对各组细胞冻后活力、回复时间长短、以及生长状况进行观察和比较,研究密度因素对细胞系L-2/M delta 2-3长期冻存效果的影响。结果表明:冻存密度对细胞系L-2/M delta 2-3冻后活力和状态恢复均有显著影响(P<0.05)。细胞冻后活力下降速率随冻存密度增高而减慢,复苏后细胞状态恢复所需时间较短。将L-2/M delta 2-3的冻存密度提高至1.3×107个.mL-1以上有利于细胞活力的保持与冻后的恢复。In order to evaluate the effects of different cell density on post-thaw cytoactives（including cell viability,recovery time,etc.） of insect cell line L-2/M delta 2-3 from embryo of Drosophila melanogaster Fallen,10 test groups with different cell density（from 0.81×106 cells·mL-1 to 2.88×107 cells·mL-1,the density span of each group was more than 2.00×106 cells·mL-1） were prepared as the cryopreserved cultures preserved in liquid nitrogen separately during 14 months.The results showed that the post-thaw viability of each test group decreased with the cryopreservation time expended.The rate of post-thaw viability decline decreased with the cell density of cryopreserved cultures increased.Cultures frozen at above 1.3×107 cells·mL-1 could obtain a better effect of cryopreservation.

关 键 词：冻存密度 冻存时间 昆虫细胞 细胞活力 

分 类 号：S789[农业科学—林学]