大黄用于抗氧化的日服用次数研究  

Research on the daily administration times of Rhubarb

作　　者：吕俊兰[1,2] 袁海龙[1] 靳世英[1,2] 付珊珊[1,2] 肖小河[3] 

机构地区：[1]中国人民解放军第三〇二医院药学部 [2]成都中医药大学药学院 [3]中国人民解放军第三〇二医院中药研究所,北京100039

出　　处：《环球中医药》2012年第3期161-164,共4页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(81073069)

摘　　要：目的采用大黄含药血清体外抗氧化效应动力学结合大黄蒽醌类成分的整合药代动力学的方法研究大黄的日服用次数。方法将24只大鼠随机分为3组,每组8只,每天分别灌服大黄提取物3.6g/kg,1次/天、1.8g/kg,2次/天和1.2g/kg,3次/天,分别于第一次给药前(0小时)及给药后5、10、15、30分钟,1、2、4、6、8、10、12、24小时眼眶后静脉取血,用于测定大鼠血中大黄酸、芦荟大黄素、大黄素、大黄酚以及大黄素甲醚五种蒽醌类成分的含量和对超氧阴离子的抑制率。计算上述成分在大鼠体内的药动学参数,利用各成分曲线下面积(AUC0-∞)百分率作为自定义权重系数,得到其整合药代动力学曲线,再与抗氧化效应动力学进行相关性分析,以此来制定大黄用于抗氧化时合理的日服用次数。结果大黄蒽醌类成分整合药代动力学与含药血清体外抗氧化效应动力学具有良好的相关性。每日给药2次组整合药代动力学AUC及抗氧化效应动力学AUC均明显高于每日给药1次和3次组。结论大黄用于抗氧化时以每日服用两次为宜。Objective To study the rational daily administration times of Rhubarb when it is used to reduce oxidative damage.Methods 24 rats were seperated into 3 groups,8 in each group,then rhubarb extract was drenched to them 3.6 g/kg once a day,1.8 g/kg twice a day and 1.2 g/kg three times a day,respectively,after they were modeled by carbon tetrachloride for 24 h.Blood collected from the veins behind the eye sockets after different intervals was used to determine the concentration of rheinic acid,aloeemodin,chrysophanol,physcion,emodin in the blood plasma and the rate of removing superoxide anion in vitro.Calculation the pharmacokinetic parameters of the above components,use the area under the curve of each component（AUC0-∞） as a percentage of custom weighting factor to get its integrated pharmacokinetic curve,then conduct correlation analysis with the antioxidant effects,determine the rational daily administration times of rhubarb for the oxidative damage.Results There was good correlation between integrated pharmacokinetics rhubarb anthraquinones and the Pharmacodynamics of antioxidant effects in vitro.The AUC of integrated pharmacokinetics and the anti-oxidation kinetics when administrated 2 times a day were significantly higher than that administrated 1 or 3 times.Conclusion It is more appropriate to administrate two times a day when Rhubarb is used as antioxidant.

关 键 词：大黄 抗氧化 整合药代动力学 效应动力学 血清药理学 日服用次数 

分 类 号：R285.6[医药卫生—中药学]