菝葜抗炎活性部位的血清药物化学研究  

Study on Serum Pharmacochemistry of Anti-inflammatory Active Site in Smilax china L.

作　　者：谢长[1] 沈婵娟[1] 陈树和[1,2] 叶晓川[1] 邹鹏程 刘焱文[1] 

机构地区：[1]湖北中医药大学中药资源与中药复方教育部重点实验室,武汉430061 [2]湖北省中医院药学部,武汉430061 [3]湖北福人药业股份有限公司产品科研部,湖北咸宁437000

出　　处：《医药导报》2012年第3期288-291,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(30870256)

摘　　要：目的对菝葜抗炎活性部位进行血清药物化学研究,探讨菝葜药效物质基础。方法建立菝葜提取物高效液相色谱(HPLC)分析法,通过HPLC图分析比较空白血清、含药血清、菝葜提取物的成分的差异,确定菝葜提取物大鼠灌胃后血中移行成分。结果在菝葜提取物含药血清中发现了45种入血成分,其中25种为原型成分,20种可能为原型成分的代谢产物。结论 45种入血成分可能是菝葜的体内直接作用物质,对其进行深入研究将有助于阐明菝葜的药效物质基础及药效机制。Objective To explore the bioactive constituents of anti-inflammatory active site of Smilax china L. for providing evidence for serum pharmacochemistry research. Methods The blood migrating constituents of Smilax china L. were determined by comparing the HPLC fingerprints of the aqueous extracts, drug contained sera and blank sera. Results Fortyfive compounds absorbed in blood were detected,including twenty-five prototype constituents of Smilax china L. and twenty metabolites. Conclusion These forty-five constituents in blood could be bioactive components of Smilax china L. , further studies on which could be useful to clarify the bioactive constituents and mechanisms of action of Smilax china L..

关 键 词：菝葜 抗炎活性部位 血清药物化学 色谱法 高效液相 

分 类 号：R282.71[医药卫生—中药学] R284[医药卫生—中医学]