H:z66抗体应激后伤寒沙门菌fliG基因缺陷株与野生株基因表达的差异  

Difference of gene expression between the wild type and fliG of Salmonella enterica serovar Typhi in response to anti-z66 antiserum stress

作　　者：张晓磊[1] 生秀梅[1] 张海方[1] 徐顺高[1] 黄新祥[1] 

机构地区：[1]江苏大学基础医学与医学技术学院生物化学研究室,江苏镇江212013

出　　处：《江苏大学学报（医学版）》2012年第1期9-13,共5页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(30870095);江苏大学高级人才启动基金资助项目(11JDG063)

摘　　要：目的:观察伤寒沙门菌鞭毛转子蛋白基因fliG在H:z66抗体应激后对其他基因表达的影响。方法:利用自杀质粒介导的同源重组法制备伤寒沙门菌fliG基因缺陷变异株;利用伤寒沙门菌全基因组芯片分析技术,比较伤寒沙门菌野生株和fliG基因缺陷变异株在H:z66抗体应激后的基因表达谱差异,并选择部分表达有差异的基因进行实时定量PCR验证。结果:经PCR验证及序列比对,伤寒沙门菌fliG基因缺陷变异株制备成功;基因表达谱比较结果表明,与野生株相比,伤寒沙门菌fliG基因缺陷变异株在H:z66抗体应激后有21个基因表达上调,7个基因表达下调;实时定量PCR结果与芯片结果一致。结论:fliG基因在伤寒沙门菌受到H:z66抗体刺激后的基因表达调控中发挥一定作用。Objective： To explore the influence of flagella rotor protein gene fliG on gene expression regulation of Salmonella enterica serovar Typhi under anti-z66 antiserum stress.Methods： The fliG deleted mutant of S.enterica serovar Typhi was prepared by homologous recombination mediated by suicide plasmid;S.enterica serovar Typhi genomic microarray was used to investigate the difference of gene expression between the wild type and fliG mutant;qRT-PCR was done to prove the results of microarray.Results： The fliG deleted mutant of S.enterica serovar Typhi was constructed successfully.Gene expression profiles analysis revealed that expression of 21 genes were increased and expression of 7 genes were decreased in the fliG mutant under anti-z66 antiserum stress.Conclusion： The flagella rotor protein gene fliG probably played a role to regulate some gene expression in response to anti-z66 antiserum stress.

关 键 词：伤寒沙门菌 FliG H:z66抗体应激 基因芯片技术 实时定量PCR 

分 类 号：R392[医药卫生—免疫学]