中国甘薯小象甲的rDNA ITS-1遗传变异及入侵来源研究  

The Study on rDNA ITS-1 Variation of Cylas formicarius (Coleoptera:Brentidae) Populations and its Invasive Sources in China

作　　者：于海滨[1,2] 沈江卫[2] 马娟[2] 马辉杰[3] 陈书龙[1,2] 

机构地区：[1]河北大学生命科学院,河北保定071000 [2]河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心,河北保定071000 [3]河北省农林科学院,石家庄050051

出　　处：《中国农学通报》2011年第18期282-287,共6页Chinese Agricultural Science Bulletin

基　　金：国家甘薯产业技术体系(CARS-11-B-08)

摘　　要：甘薯小象甲是国内外重要检疫性害虫,明确中国不同地区甘薯小象甲的遗传多样性可为甘薯小象甲的鉴别检疫提供依据。通过对中国6个地区甘薯小象甲种群rDNAITS-1序列的遗传变异分析探讨了中国6个地区种群的入侵来源。结合世界其他地区已公布35个甘薯小象甲种群序列,经系统发育分析表明,甘薯小象甲起源于印度次大陆,在亚洲地区随甘薯的调运而自东南向西北扩展,构建的系统发育树分为印度地区分支和亚洲东部分2个大的分支,而亚洲东部分支又进一步划分为东南部和东北部2个亚支,中国的甘薯小象甲种群分别位于东北部分支的2个亚分支上,说明中国6个地区甘薯小象甲种群至少通过2个不同地区侵入中国,并在中国定居。Sweet potato weevils （Cylas formicarius） are important quarantine pest in the world.Understanding the genetic diversity of sweet potato weevils in different areas of China can provide the basis for its identification and quarantine.Genetic variations of the ITS-1 region were examined to probe the invasive sources for 6 populations from different areas in Southern China.Phylogenetic analysis on those sequences together with 35 known C.formicarius sequences showed that sweet potato weevil originated in India,then spread all over Asia from southeast to northwest along with the transportation of sweet potatoes.The phylogenetic tree consisted of two main clades （India and East Asia）.The East Asia clade was further divided into two subclades （Northeast and Southeast）.Chinese populations were all in two subclades of the Northeast subclade.It suggested that the 6 populations in China might come from the two origins and settled in China.

关 键 词：甘薯小象甲 ITS-1 系统发育树 侵入来源 

分 类 号：S40[农业科学—植物保护]