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机构地区:[1]肇庆学院果树研究所,广东肇庆526061 [2]广东省农业科学院果树研究所,广州518040
出 处:《中国农学通报》2011年第19期222-227,共6页Chinese Agricultural Science Bulletin
基 金:广东省科技计划项目(2010B080201006);肇庆市科技计划项目(2009N029)
摘 要:利用随机扩增多态性DNA(RAPD)技术,研究了地方优良品种‘贡柑’与部分柑橘属植物之间的亲缘关系,10条引物共扩增出87条谱带,多态性谱带率为97.7%,通过各供试材料间遗传相似系数建立的聚类树状图,能准确地区分各种、品种、品系的关系,此外对‘贡柑’的起源进行了初步的探讨,支持‘贡柑’是由本地橘和橙的自然杂交而来的观点,并偏向橘遗传基础。The phylogenetic relationships among Citrus reticulata ‘ Gonggan ’ and partial Citrus were investigated by random amplified polymorphism DNA (RAPD). 10 primers generated 87 bands, with an average of 97.7% polymorphic bands. Dendrogram was drawn by the genetic similarity among the materials tested, which could help to clearly distinguish the relationships among different species, breeds and lines. The genetic background of Citrus reticulata ‘ Gonggan ’ was analyzed primarily as well, the results supported the view that Citrus reticulata ‘ Gonggan ’ was a natural hybrid of orange and tangerine more emphasis on tangerine which originating from Sihui region.
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