PreS1抗原与HBeAg联合检测用于预测HBV-DNA的水平  

Prediction of HBV-DNA level by co-detection of serum PreS1 and HBeAg in chronic HBV-carriers

作　　者：李珉珉[1] 洪丹妮[1] 朱勤爱[1] 朱穗兰[1] 

机构地区：[1]暨南大学附属第一医院临床检验中心,广东广州510630

出　　处：《暨南大学学报（自然科学与医学版）》2011年第6期637-640,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省医学科研基金项目(A2010327)

摘　　要：目的:联合检测慢性乙型肝炎病毒(HBV)携带者的血清前S1(PreS1)抗原和乙肝e抗原(HBeAg)两项指标,判断其与HBV-DNA定量的相互关系。方法:采用双抗体夹心酶联免疫吸附分析(ELISA)法(两步法)检测PreS1抗原,双抗体夹心ELISA法(一步法)检测HBeAg,实时荧光定量聚合酶链反应(PCR)法检测HBV-DNA。结果:在335例慢性HBV携带者患者中,PreS1抗原阳性率为42.7%,HBeAg阳性率为36.4%,HBV-DNA阳性率为53.4%。在179例HBV-DNA阳性患者中,HBeAg阳性109例(60.9%);156例HBV-DNA阴性患者中,HBeAg阴性143例(91.7%)。在179例HBV-DNA阳性患者中,PreS1抗原阳性89例(49.7%);在156例HBV-DNA阴性患者中,PreS1抗原阴性102例(65.4%)。PreS1和HBeAg双阳性的感染者HBV-DNA阳性率为93.8%(45/48);在PreS1和HBeAg双阴性的感染者中,HBV-DNA阳性率仅为22.0%(26/118)。结论:HBeAg与HBV-DNA的相关性比PreS1抗原与HBV-DNA的相关性高。PreS1和HBeAg双阳性的感染者HBV的复制程度高,而双阴性的感染者复制程度较低。因此,PreS1抗原与HBeAg联合检测可用于预测HBV-DNA的水平。Aim：To study the relationship of HBV-DNA with the HBV biomarkers PreS1 and HBeAgin chronic HBV-carriers. Methods： PreS1 and HBeAg were detected by sandwich ELISA. HBV-DNAwas detected by fluorescence quantitative polymerase chain reaction assay. Results： Among 335 chronicHBV-carriers＇serum samples,the positivety rate of preS1, HBeAg and HBV-DNA were 42.7%, 36. 4%and 53.4%, respectively. Meanwhile, the positivety rates of preS1 and HBeAg in patients with serumHBV-DNA positive were 49.7% （89/179） and 60. 9% （109/179）, respectively. The negative rates ofpreS1 and HBeAg in patients with serum HBV-DNA negative were 65.4% （102/156） and 91.7%（143/156）, respectively. The positive rates of serum HBV-DNA in patients with PreS1 and HBeAg bothpositive were 93.8% （45/48）, while only 22.0% （26/118） in patients with PreS1 and HBeAg bothnegative. Conclusion：There is a high correlation between HBeAg and HBV-DNA, indicating that HBeAghas a better concordance with HBV-DNA than PreS1. HBV carriers with PreS1 and HBeAg both posi-tivety have high HBV-DNA copies. The co-detection of PreS1 and HBeAg can predict the replication lev-el of HBV.

关 键 词：慢性HBV携带者 PRES1抗原 乙肝病毒DNA 乙肝E抗原 

分 类 号：R512.6[医药卫生—内科学] R446.6[医药卫生—临床医学]