国内外云计算研究的现状与发展——基于INSPEC数据库的分析  

Effects of Nicotine Analog ZY-1 on β-amyloid Production and Learning and Memory in Transgenic Alzheimer Disease Mice

作　　者：聂惠贞[1] 王泽剑[1] 赵文娟[1] 卢金淼[1] 张璨[1] 骆庆和[1] 王玉亮[1] 高翔[2] 殷明[1] NIE Hui-zhen1,WANG Ze-jian1,ZHAO Wen-juan1,LU Jin-miao1,ZHANG Can1,LUO Qing-he1,WANG Yu-liang1,GAO Xiang2,YIN Ming1 1.？School of Pharmacy,Shanghai Jiaotong University,Shanghai,200240,China 2.？Model Animal Research Center,Nanjing University,Nanjing,210061,China

机构地区：[1]上海交通大学药学院,中国上海200240 [2]南京大学模式动物研究所,中国南京210061

出　　处：《神经药理学报》2011年第1期26-31,共6页Acta Neuropharmacologica

基　　金：上海市科委生物医药科技攻关重点基金（No.08431900600）; 第四十五批中国博士后科学基金（No.20090450709）; 综合性新药研究开发技术大平台（No.2009ZX09301-007）; 闵行区-上海交通大学区校合作专项基金（No.2009003）

摘　　要：运用文献计量方法,通过科学文摘数据库检索1969年至今所有云计算的文献,并从文献量、著者、期刊和关键词等方面对云计算领域研究论文进行分析,从而探讨世界范围内的云计算研究的现状与发展趋势。Objective：To observe the effects of ZY-1,a nicotine analogue,on in vitro receptor binding and β-amyloid（Aβ） production and in vivo transgenic Alzheimer＇s disease mice.Methods： Receptor binding assay was carried out in SH-EP1-α4β2nAChR cell line highly expressing α4β2nAChR;Western blotting was used to detect the effects of ZY-1 on amyloid protein production in the SH-EP1-α4β2nAChR cell line transfected with APP695 plasmid;and effects of ZY-1 on learning and memory were measured in B6C3-Tg（APPswe,PSEN1dE9）85 Dbo/J mice.Results：①Receptor binding assay showed that Ki is 21.5 nmol·L-1,suggesting specific binding of ZY-1 to α4β2nAChR;②ZY-1（1 μmol·L-1）reduced intracellular production of Aβ;③Middle（0.50 mg·kg-1） and high dose（0.75 mg·kg-1） of ZY-1 improved learning and memory of transgenic mice in Morris water maze and platform test.Conclusion： ZY-1 might be of potential to be an AD therapeutic agent,but the druggability remains to be further confirmed.

关 键 词：云计算 INSPEC数据库 定量分析 

分 类 号：R964[医药卫生—药理学;医药卫生—药学]