莫诺苷对二磷酸腺苷诱导兔血小板聚集钙离子的影响  

Morroniside Inhibiting Ca2 + after Platelet Aggregation Induced by Adenosine Diphosphate in Rabbits

作　　者：艾厚喜[1] 左玮[1,2] 王晓锋[2,3] 张丽[2] 季晖[2] 陈乃宏[3] 王文[1] 

机构地区：[1]首都医科大学宣武医院,北京市100053 [2]中国药科大学,江苏南京市210009 [3]中国医学科学院药物所,北京市100050

出　　处：《中国康复理论与实践》2012年第2期131-133,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：“重大新药创制”科技重大专项(2009ZX09103-366);国家自然科学基金项目(30973893);北京市自然科学基金项目(7102077);北京市教委(PXM2011_014226_07_000071);2011年北京市卫生系统高层次卫生技术人才

摘　　要：目的观察莫诺苷对二磷酸腺苷(ADP)诱导兔血小板聚集后Ca2+浓度的影响。方法利用钙离子荧光探针(Fura-2 AM)法,通过记录5 min之内Fura-2在激发波长340 nm和380 nm处的荧光强度比值,检测不同条件下ADP诱导血小板聚集后Ca2+的变化。结果与空白对照组相比,莫诺苷能显著抑制由ADP诱导兔血小板聚集后Ca2+的升高(P<0.001)。结论莫诺苷可能通过降低Ca2+的上升达到抗ADP诱导的兔血小板的聚集,从而改善体外血液流变学。Objective To explore the effects of morroniside on Ca2＋ in the condition of platelet aggregation in rabbits induced by adenosine diphosphate（ADP）.Methods The mobilization of cytosolic-free calcium after platelet aggregation induced by ADP was detected by Ca2 ＋-sensitive fluorescent indicator,Fura-2 AM and time scan measurement.Results Compared with the controls,morroniside significantly inhibited the increase of Ca2＋ induced by ADP（P〈0.001）.Conclusion Morroniside acts as an effective platelet aggregative antagonist by inhibiting the increase of platelet Ca2＋.

关 键 词：莫诺苷 血小板聚集 钙 兔 

分 类 号：R285.5[医药卫生—中药学]