原发性肾病综合征患儿外周血CD4^+CD_(25)^+调节性T细胞及CD_(19)^+CD_(23)^+细胞水平的变化及其意义  

Changes and Significance of CD4^+CD_(25)^+ Regulatory T Cells and CD_(19)^+CD_(23)^+ Cells in Children with Primary Nephrotic Syndrome

作　　者：毕玉娜[1] 禚金花[2] 尹立岩[1] 王利珍[1] 李晓忠[3] 季正华[4] 

机构地区：[1]山东省交通医院儿科,济南250031 [2]山东省交通医院检验科,济南250031 [3]苏州大学附属儿童医院肾脏免疫科,江苏苏州215003 [4]苏州大学附属儿童医院检验科,江苏苏州215003

出　　处：《实用儿科临床杂志》2012年第5期349-350,353,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(30972711);江苏省自然科学基金(BK2009128)

摘　　要：目的通过观察原发性肾病综合征(PNS)患儿外周血淋巴细胞亚群,尤其是CD4+CD25+调节性T细胞及CD19+CD23+细胞水平的变化,探讨其免疫发病机制。方法采用双标法用流式细胞仪检测25例初发PNS患儿(PNS组)外周血T淋巴细胞亚群(CD3+、CD3+CD4+、CD3+CD8+、CD4+CD25+)、B淋巴细胞亚群(CD3-CD19+、CD19+CD23+)及自然杀伤细胞(CD3-CD16+56+)水平,同时选取同期19例健康儿童作为健康对照组。数据采用SPSS 15.0软件进行统计学分析。结果 PNS组患儿外周血中CD3+、CD3+CD8+、CD4+CD25+、CD19+CD23+淋巴细胞均显著高于健康对照组(Pa<0.05),而自然杀伤细胞则较健康对照组显著降低(P<0.05);PNS组CD3+CD4+、CD4+/CD8+、CD3-CD19+淋巴细胞与健康对照组比较差异无统计学意义。结论体内淋巴细胞亚群的紊乱参与了PNS的发病过程,其中CD4+CD25+调节性T细胞及CD19+CD23+细胞的变化为PNS的免疫治疗目标提供了理论依据。Objective To investigate the immunological mechanism of the primary nephrotic syndrome（PNS） and analyze the changes of peripheral-blood lymphocyte subsets in PNS children,especially the CD4＋CD25＋ regulatory T cells and the CD19＋CD23＋ cells. Methods Flow cytometry technique was applied to detect the T-lymphocyte,B-lymphocyte and natural killer cells in 25 cases of PNS children（PNS group） and 19 healthy children（healthy control group）.SPSS 15.0 software was used to analyze the data. Results The frequencies of CD3＋,CD3＋CD8＋,CD4＋CD25＋ regulatory T cells,as well as CD19＋CD23＋ lymphocytes were all increased more remarkably in PNS group than those in healthy control group（Pa0.05）,whereas the number of natural killer cells were reduced（P0.05）.Moreover,there were no significant differences of CD3＋CD4＋,CD4＋/CD8＋ and CD3-CD19＋ lymphocytes in two groups. Conclusions Disturbance of lymphocyte subsets is involved in the pathogenesis of PNS.Thus,there can be a potentially effective immunological strategy for the treatment of PNS in children by properly regulating the expressions of CD4＋CD25＋ regulatory T cells and CD19＋CD23＋ lymphocyte.

关 键 词：原发性肾病综合征 CD4+CD25+ CD19+CD23+ 流式细胞仪 

分 类 号：R726.91[医药卫生—儿科]