阿托伐他汀对ApoE^(-/-)小鼠动脉粥样硬化模型中血清一氧化氮、肿瘤坏死因子-α表达的影响  

Effects of atorvastatin on the serum NO and TNF-α in ApoE^(-/-) mice for atherosclerosis

作　　者：张敏[1] 宁亚媛[2] 陈丽娟[2] 王珺楠[2] 

机构地区：[1]吉林大学第二医院急救医学科,长春市130041 [2]吉林大学第二医院心血管内科,长春市130041

出　　处：《实用医学杂志》2012年第5期715-717,共3页The Journal of Practical Medicine

基　　金：吉林省科技发展计划项目(编号:20070720-3)

摘　　要：目的:探讨阿托伐他汀抗动脉粥样硬化的机制。方法:24只载脂蛋白E基因敲除小鼠随机分为3组(n=8),分别给予普通饮食(空白对照组)、高脂饮食(高脂饮食组)、高脂饮食+阿托伐他汀(高脂他汀组),建立动脉粥样硬化模型,人工饲养8周后检测各组小鼠血清一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)水平及行小鼠主动脉弓HE染色。结果:(1)HE染色结果显示:空白对照组主动脉内膜未见明显增厚,泡沫细胞含量极少;高脂饮食组小鼠主动脉弓可见明显动脉粥样硬化斑块,内膜明显增厚,其内可见大量泡沫细胞及增生的纤维组织,胞体大,胞浆呈空泡状,纤维帽薄;高脂他汀组主动脉亦可见明显动脉粥样硬化斑块,内膜稍增厚,泡沫细胞含量极少,可见较厚纤维帽。(2)普通饮食组、高脂饮食组、高脂他汀组血清NO分别为(56.104±16.104)、(29.881±12.009)、(53.713±16.430)μmol/L,TNF-α分别为(3.138±0.435)、(5.163±0.523)、(3.938±0.452)pg/mL(P<0.05)。结论:已知NO及TNF-α与动脉粥样硬化相关,阿托伐他汀能够调节小鼠血清NO、TNF-α的表达,提示阿托伐他汀能够通过调整炎性介质的表达发挥其抗动脉粥样硬化的作用。Objective To investigate antiatherosclerotic mechanism of atorvastatin. Methods Twenty-four ApoE-/- mice were randomly divided into three groups （n = 8）, and given normal diet, high-fat diet, high-fat diet add atorvastatin, to establish atherosclerosis model. After 8 weeks, the serum level of NO, TNF-α were detected and aortic arch were stained by HE staining. Results （1）HE staining showed： In the control group, aortic intima was non-thickened, foam cell was poor. High-fat diet group showed obvious atherosclerotic plaque in aortic arch, thickened intima, a large number of foam cells, hyperplastic fibrous tissue, large soma, cytoplasmic vacuole and thin fibrous cap. High-fat diet add atorvastatin group also showed significant atherosclerotic plaque, slightly thickened intima, few foam cells and thick fibrous cap. （2）The serum levels of NO in three groups were （56.104 ± 16.104）, （29.881 ± 12.009）, （53.713 ± 16.430）μmol/L, and TNF-α were （3.138 ± 0.435）, （5.163 ± 0.523）, （3.938 ± 0.452）pg/mL （P 0.05）, respectively. Conclusion Atorvastatin can regulate the serum levels of NO and TNF-α, which are associated with atherosclerosis. It suggests that atorvastatin adjust the expression of inflammatory mediators and play an important role in anti-atherosclerosis.

关 键 词：动脉粥样硬化 阿托伐他汀 血清NO 血清TNF-Α 

分 类 号：R543.5[医药卫生—心血管疾病]