开口箭根茎中甾体类化合物的研究  

Isolation and Structural Identification of Spirostanol Compounds from Tupistra Chinensis

作　　者：邬昊洋[1] 王倩[1] 刘呈雄[1] 邹坤[1] 

机构地区：[1]三峡大学化学与生命科学学院天然产物研究与利用湖北省重点实验室,湖北宜昌443002

出　　处：《三峡大学学报（自然科学版）》2012年第1期85-88,共4页Journal of China Three Gorges University：Natural Sciences

基　　金：国家自然科学基金项目(30870254;31070313;30670213);湖北省自然科学基金创新群体项目(2007ABC008)

摘　　要：目的:研究开口箭根茎中甾体成分,寻找新的抗肿瘤活性物质.方法:运用反相常压柱色谱和制备高效液相对其甾体成分进行分离,通过理化性质和波谱分析方法鉴定其结构.结果:从开口箭根茎的乙醇提取物中分离得到6个甾体皂苷及2个甾体皂苷元,其结构分别为1β,2β,3β,4β,5β-五羟基-螺甾-△25(27)-烯-5-O-β-D-吡喃葡萄糖苷(1),1β,3β-二羟基-5β-孕甾-△16(17)-烯-20-酮-3-O-β-D-吡喃葡萄糖苷(2),1β,2β,3β,4β,5β,6β,7α-七羟基-5β-呋甾-△25(27)-烯(3),1β,2β,3β,4β,5β,7α-六羟基-6-氧化-螺甾-△25(27)-烯(4),3-O-β-D-吡喃葡萄糖基-(25S)-1β,3β,5β,26-四羟基-5β-呋甾-△20(22)-烯-26-O-β-D-吡喃葡萄糖苷(5),3-O-β-D-吡喃葡萄糖基-(25S)-1β,3β,5β,22α,26-五羟基-5β-呋甾-26-O-β-D-吡喃葡萄糖苷(6),3-O-β-D-吡喃葡萄糖基-(25S)-1β,3β,26-三羟基-5β-呋甾-26-O-β-D-吡喃葡萄糖苷(7)和3-O-β-D-吡喃葡萄糖基-(25R)-1β,3α,26-三羟基-△5(6)-烯-5β-呋甾-26-O-β-D-吡喃葡萄糖苷(8).结论:化合物1和2为首次从该属植物中获得.To study the steroidal saponins from the fresh rhizomes of Tupistra chinensis and search for bioactive saponins with potential antitumor activity.The steroidal saponins were isolated by RP-C18 column chromatography and preparative HPLC.The structures of the compounds were dertermined on the basis of the spectroscopic analysis and chemical evidences.Eight spirostanol compounds（1-8） were obtained from the n-butanol fraction of EtOH extract from Tupistra chinensis rhizomes.Their structures were determined on the basis of chemical methods and spectral data.Compounds 1 and 2 were isolated from this plant for the first time.

关 键 词：开口箭 甾体皂苷 呋甾皂苷 螺甾烯 

分 类 号：R284[医药卫生—中药学]