小花棘豆(Oxytropis glabra)内生真菌固体培养条件优化研究  

Optimization of Solid Culture Conditions of Fungal Endophytes Isolated from Oxytropis glabra

作　　者：何珊[1] 卢萍[1] 包睿媛[1] 

机构地区：[1]内蒙古师范大学生命科学与技术学院.内蒙古呼和浩特010022

出　　处：《内蒙古师范大学学报（自然科学汉文版）》2012年第1期105-108,共4页Journal of Inner Mongolia Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30860049)

摘　　要：从采自内蒙古鄂尔多斯市的小花棘豆中分离得到内生真菌,研究菌株OW 5.3的优化固体培养条件.在不同碳源、初始pH条件下,检测内生真菌的生长速度,并利用气相色谱技术测定内生真菌菌丝体中苦马豆素的含量.结果显示:菌株OW5.3最适起始pH为7.0;以葡萄糖为碳源时生长速度较快;以燕麦片为碳源时,菌丝体的苦马豆素含量最高.Fungal endophytes isolated from Oxytropis glabra of Ordos,Inner Mongolia were cultured and optimizing culture conditions of the strain OW 5.3 in solid medium were studied.Detecting the growth speeds and determining the swainsonine producing levels of the fungal endophytes by gas chromatography at different culture conditions,such as different carbon sources and different initial pH values showed the following results：when the initial pH value was at 7.0,the growth speed was the fastest and the content of swainsonine in hyphae was the highest;The growth speed was faster when the carbon source was glucose;The level of swainsonine in hyphae was highest when the medium was oatmeal.

关 键 词：小花棘豆 内生真菌 优化培养 苦马豆素 

分 类 号：Q948.122.3[生物学—植物学]