ELISA检测IL-1α的实验优化研究  

Optimizing Reaction Methods for ELISA Detecting human IL-1α

作　　者：张丽娜[1,2] 余平[1] 程文[1] 

机构地区：[1]中南大学基础医学院,410000 [2]长治医学院基础部免疫学教研室

出　　处：《长治医学院学报》2012年第1期13-15,共3页Journal of Changzhi Medical College

基　　金：国家自然科学基金(81071403)

摘　　要：目的:研究酶联免疫吸附试验(ELISA)检测白细胞介素1(IL-1α)的最佳试验条件,用优化配制的TMB/HRP显色体系最大程度降低成本,可适用于多数ELISA测定。方法:以前期优化方案为基础,进一步对抗体包被时间、包被温度、显色液配制等条件的优化,检测IL-1α标准品OD值并进行结果比较。结果:抗体包被于室温24h,最优显色体系为按四甲基联苯胺(TMB)终浓度0.1mg/mL和0.03%H2O2配制,可获得最佳IL-1α定量结果。结论:用优化后的ELISA方法测定IL-1α的敏感性、重复性及标准曲线拟合度更佳,具有良好的应用前景。Objective：To optimize reaction conditions for ELISA detecting human IL-lα. Methods：The constitution of coating time, incubation time and the TMB/HRP substrate chromogenic system were optimized as well to examine the standard samples of human IL-lα. Results： By extending coating time to 24 h in room temperture and the substrate chromogenic system was prepared by TMB dissolved in 0.1 mg/mL and 0.03 % hydrogen peroxide urea, quantitative ELISA for human IL-lα assay had better results. Conclusion： By optimize the sensitivity, repatition and standard curve of the quantitative ELISA for human IL-lα is better. Therefore,the application value and potential was very large.

关 键 词：酶联免疫吸附法试验 IL-1Α 试验条件 

分 类 号：R446.61[医药卫生—诊断学]