甲醛对人外周血淋巴细胞遗传毒性的研究  

Studies on Genotoxicity of Formaldehyde on Human Peripheral Lymphocyte

作　　者：马红莲[1] 霍静[1] 张联珠[1] 边肖海[2] 倪春娟[1] 

机构地区：[1]长治医学院生物学教研室,046000 [2]长治医学院附属和平医院妇产科

出　　处：《长治医学院学报》2012年第1期19-21,共3页Journal of Changzhi Medical College

摘　　要：目的:研究甲醛对人外周血淋巴细胞染色体的影响。方法:用不同浓度(5、25、125、500μmol/L)的甲醛溶液处理培养中的人外周血淋巴细胞,观察其微核率及SCE频率的变化。结果:低浓度(5、25μmol/L)甲醛处理后,人外周血淋巴细胞微核率和SCE频率与阴性对照组相比,无明显增加(P>0.05);而甲醛浓度(125、500μmol/L)较高时,细胞微核率和SCE频率均显著高于阴性对照组(P<0.05或P<0.01)。结论:较高浓度甲醛对人外周血淋巴细胞染色体有不同程度的损伤作用。Objective：Effects of formaldehyde on peripheral lymphocyte chromosome out of human body was studied. Methods：Lymphocytes in culture fluid were treated by supplementing different doses （5, 25,125, 500 /通讯作者mol/L） of formaldehyde,then the changes of micronucleus and SCE rates were observed. Results： It is found that no significant increment in both Mieronucleus and SCE rates were detected at low doses of formaldehyde（5,25/μmol/L）in peripheral lym- phocytes（ P 〉0.05） ;Micronucleus and SCE rates of test group were obviously higher than those of the negative control（ P 0.05 or P 〈0.01） at the higher doses （125,500 μmol/L）. Conclusion： formaldehyde may exert different degree of damage to peripheral lymphocyte chromosome out of human body.

关 键 词：甲醛 遗传毒性 人外周血淋巴细胞 

分 类 号：R136.3[医药卫生—劳动卫生]