高效液相色谱法测定癸氧喹酯原料及制剂含量的研究  

Study on Determination of Decoquinate and Its Preparation by HPLC

作　　者：姜富成[1] 刘聚祥[1] 王红霄 王克宁[1] 王建平[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]华北制药集团动物保健品有限责任公司,石家庄050041

出　　处：《中国兽药杂志》2012年第3期40-43,共4页Chinese Journal of Veterinary Drug

摘　　要：建立了癸氧喹酯原料药、固体分散体含量测定的高效液相色谱-紫外检测法。采用Diamonsil C18(4.6 mm×150 mm,5μm)反相色谱柱,以甲醇-水(95∶5)溶液(用稀盐酸调pH值至3.5)为流动相,检测波长330 nm,流速0.6 mL/min,外标法定量。结果显示该方法最低检测限为0.0025μg/mL,在0.5～10μg/mL浓度范围内,线性关系良好(A=11405C+15821,R2=0.999),加样平均回收率99.61%,RSD为0.90%。此方法可用于癸氧喹酯的含量测定,适用于该药品固体分散体生产的质量控制。A detection method of HPLC - UV was established for determination of decoquinate and its solid dispersion preparation. Diamonsil C18 column （4.6 mm x 150 mm,5 μm） was used with the mobile phase of methanol-water（95 ： 5） solution（to adjust pH value of 3.5 by dilute hydrochloric acid）, The UV detection wavelength was 330 nm with flow rate of 0.6 mL/min. The quantification was carried out by the external standard method. Results showed that the limit of detection method was 0. 0025 p,g/mL. The calibration curves had good linearity within the concentration range of 0.5 - 10 μg/mL with the correlation coefficient of 0. 999 （ n = 6）. The average recovery of deeoquinate was 99.61% with RSD of 0.90% （ n = 9 ）. The all above - mentioned results indicated that the HPLC method can be used for content determination of deeoquinate and was applicable to product quality control of its solid dispersion preparation.

关 键 词：高效液相色谱法 癸氧喹酯 含量测定 

分 类 号：R927.2[医药卫生—药学]