MSP对COPD大鼠肺泡巨噬细胞氧化应激和细胞因子产生的影响  

Effect of Macrophage-stimulating Protein on the Release of Cytokine and Oxidative Stress of Alveolar Macrophages in COPD Rats

作　　者：陈小菊[1] 王涛[1] 陈心容[1] 陈小兵[1] 

机构地区：[1]川北医学院附属医院呼吸内科,四川南充63700

出　　处：《求医问药（下半月刊）》2011年第12期172-173,共2页Seek Medical and Ask The Medicine

基　　金：四川省卫生厅科研项目(070293)

摘　　要：目的:探讨巨噬细胞刺激蛋白(MSP)对COPD大鼠肺泡巨噬细胞氧化应激和细胞因子产生的影响。方法;培养正常和COPD大鼠肺泡巨噬细胞,予不同浓度MSP干预,采用酶联免疫法检测细胞上清液中细胞因子TNF-α、IL-8、IL-1β和IL-10的浓度,比色法检测细胞上清液中丙二醛(MDA)和超氧化物歧化酶(SOD)的水平。结果:经MSP处理后,正常大鼠和COPD大鼠肺泡巨噬细胞分泌TNF-α、IL-8、IL-1β和IL-10呈浓度依赖性增加,而COPD大鼠肺泡巨噬细胞上清液中TNF-α、IL-8和IL-1β浓度较正常大鼠增加更显著。MSP呈浓度依赖性刺激正常和COPD大鼠肺泡巨噬细胞分泌MDA增加,抑制其SOD的产生,而COPD大鼠肺泡巨噬细胞上清液中SOD水平较正常大鼠降低更加明显。结论:MSP能显著增加COPD大鼠肺泡巨噬细胞分泌TNF-α、IL-8和IL-1β,抑制其产生SOD。提示MSP在COPD的发病机制中可能起重要作用。Objective To investigate the effect of mcrophage-stimulating protein（MSP）on the release of cytokine and oxidative stress of alveolar macrophage（sAms）in COPD rats.Methods Cultured normal and COPD rats Ams were treated with different concentrations of MSP.The levels of TNF-α、IL-8、IL-1β and IL-10 in the supernatant of AMs were determined with ELISA.The levels of maleic dialdehyd（MDA）and superoxide dismutase（SOD） in the supernatant of AM were assayed by chromatometry method.Results MSP increased TNF-α、IL-8、IL-1β and IL-10 levels in the supernatant of AMs from normal and COPD rats in a concentration-dependent manner.The levels of TNF-α、IL-8 and IL1β were significantly increased in copd rats Ams,as compared to normal rats.MSP increased the production of MDA and inhibited the production of SOD in a concentration-dependent manner.The level of SOD was significantly decreased in copd rat Ams,as compared to normal rats.Conclusion MSP increases significantly the release of TNF-α、IL-8、IL-1β and inhibits the production of SOD in cultured COPD rats AMs.It is indicated that MSP plays important role in the pathogenesis of COPD.

关 键 词：MSP 肺泡巨噬细胞 细胞因子 氧化应激 COPD 

分 类 号：R861.41[医药卫生—临床医学]