超声联合一氧化氮微泡体外干预骨髓间充质干细胞安全性探讨  

Investigation of the safety of ultrasound combined with nitric oxide microbubbles in vitro intervening mesenchymal stem cells

作　　者：卞叶萍[1] 李鹏[2] 童嘉毅[1] 杨芳 陈龙[1] 王庆捷[1] 宋佳贤[1] 沈祥波[2] 姚玉宇[2] 马根山[1] 

机构地区：[1]东南大学附属中大医院心脏病科,江苏省210009 [2]东南大学医学院 [3]江苏省生物材料与器件重点实验室

出　　处：《江苏医药》2012年第5期500-502,共3页Jiangsu Medical Journal

基　　金：国家自然科学基金(81070265/H0222)

摘　　要：目的研究超声联合一氧化氮(NO)微泡体外干预大鼠骨髓间充质干细胞(BMSCs)的安全性。方法体外分离培养大鼠BMSCs,分为超声联合NO微泡组(包括1∶70,1∶50,1∶20浓度亚组)及空白对照组,体外干预后MTT法检测BMSCs增殖,PI染色流式细胞术分析细胞凋亡及细胞周期。结果与空白对照组比较,1∶20浓度组显著抑制BMSCs增殖(P<0.05);1∶50浓度组与1∶20浓度组明显诱导细胞凋亡(P<0.05)。结论超声联合NO微泡体外干预BMSCs,1∶70浓度对其是安全的。Objective To study the safety of ultrasound combined with nitric oxide（NO） microbubbles in vitro intervening mesenchymal stem cells（BMSCs）.Methods Rat bone marrow BMSCs isolated and cultured in vitro were randomly divided into the control（group C） and the ultrasound combined NO microbubble intervention groups of Ia（1∶70）,Ib（1∶50） and Ic（1∶20）.The proliferation of BMSCs was detected by MTT assay and the apoptosis and cell cycle were measured by flow cytometry after PI dyeing.Results Compared to group C,the proliferation of BMSCs in group Ic was significantly inhibited（P0.05） and the induced apoptosis was more in groups of Ib and Ic（P0.05）.Conclusion The ratio of ultrasound to NO microbubble 1∶70 is safe to intervene BMSCs.

关 键 词：超声 一氧化氮微泡 骨髓间充质干细胞 

分 类 号：R541[医药卫生—心血管疾病]