江苏汉族群体15个STR基因座遗传多态性分析  

A genetic polymorphism analysis on fifteen short tandem repeat loci among Han population in Jiangsu district

作　　者：潘猛 陈子庆[1,2] 丁小健 居晓斌[1,2] 陈奇 周蓉[1,2] 周惠英 

机构地区：[1]南京医科大学第一附属医院 [2]江苏省人民医院司法鉴定所,江苏省210029

出　　处：《江苏医药》2012年第5期561-564,共4页Jiangsu Medical Journal

摘　　要：目的分析15个短串联重复序列(STR)基因座在江苏汉族群体中的基因多态性和基因频率。方法取2010年-2011年来自江苏省的1000例无血缘关系的汉族个体。用Sinofiler 16荧光标记复合扩增系统15个STR位点进行扩增,自动基因分析仪片段分析并进行基因分型。结果累积STR等位基因共发现211个,其中罕见等位基因36个。基因型频率超过0.1的基因座型22个,其中D3S1358基因座的15/16等位基因型频率最高达到0.2225。杂合度达到了0.802±0.051,随机个体相同表型偶合率值为0.068±0.031,多态信息总量值为0.778±0.060,个体识别能力和非父排除率值分别为0.932±0.032和0.607±0.091。FGA、D12S391基因座在发生24次突变的11个基因座中有较高的突变率。结论通过大量的样本实验和等位基因频率总结,得到了江苏人群更多法医学亲权鉴定和个人识别等位基因多样性的客观数据。Objective To analyze the genetic polymorphism and frequencies of 15 short tandem repeat（STR）loci in 1000 unrelated individuals of Han nationality in Jiangsu province.MethodsFifteen STR loci were amplified in DNA samples from 1000 unrelated individuals by Sinofiler 16 System in Jiangsu Han population from 2010 to 2011.All samples of the PCR were genotyped by ABI 3130 Genetic Analyzer.Results A total of 211 genotypes was obtained,of which the numbers of rare alleles were 36.The numbers of frequency of greater than 0.1 were 22,of which the maximum frequency of ＂15/16＂ genetype from D3S1358 locus was 0.2225.Hets was 0.802±0.051,Pm was 0.068±0.031,PIC was 0.778±0.060,DP was 0.932±0.032 and PE was 0.607±0.091.FGA and D12S391 loci with 24 events had a higher rate of mutation at 11 loci.Conclusion With the analysis of alleles in large number of samples and frequencies,objective alleles geneticpolymorphism data about paternity testing and personal identification have been obtained among Jiangsu Han population

关 键 词：亲权鉴定 基因多态性 短串联重复序列 

分 类 号：Q343[生物学—遗传学]