牛带绦虫成虫谷胱甘肽S-转移酶基因的生物信息学分析  

Bioinformatics Analysis of Glutathione S-transferase Gene of Taenia saginata

作　　者：王宇[1] 黄江[1] 戴佳琳[2] 廖兴江[2] 

机构地区：[1]贵阳医学院寄生虫学教研室,贵州贵阳550004 [2]贵阳医学院多媒体形态学实验室,贵州贵阳550004

出　　处：《贵阳医学院学报》2012年第1期5-8,共4页Journal of Guiyang Medical College

基　　金：国家自然科学基金资助项目(30760227);贵州省科技攻关项目[黔科合NY字(2008)3060]

摘　　要：目的:分析牛带绦虫成虫谷胱甘肽S-转移酶(GST)基因结构并预测其编码蛋白的结构和功能。方法:利用生物信息学网站如NCBI和ExPASY系统中的生物信息学分析工具,并结合其它分析软件,分析该基因的结构并预测其编码蛋白质的结构和功能。结果:该基因全长908 bp,编码区为135～771 bp,编码212个氨基酸,无各种亚细胞定位序列;与猪带绦虫GST的一致性为93%,相似性为96%;预测3个主要的抗原表位33～53 aa,62～68 aa,179～184 aa位于空间结构上相距较远的分子表面。结论:从牛带绦虫成虫cDNA文库中筛选出GST基因,预测为胞浆型蛋白,可能具有较好的免疫学诊断抗原应用前景。Objective： To analyze gene structure of glutathione S-transferase（GST） of Taenia saginata,and to predict the structure and function of its encoded protein.Methods： Bioinformatics analysis tools in bioinformatics webs such as NCBI and ExPASY combined with some other analysis softwares were used.Results： The full length of this gene was 908 bp.Its coding region was 135～771 bp,encoding 212 amino acids.The encoded protein didn′t contain any kinds of subcellular localization sequence.Consistency and similarity of the screened gene with that of Taenia solium GST were 93% and 96% respectively.Three major epitopes of GST： 33～53 aa,62～68 aa,179～184 aa were predicted to locate on the surface of GST spatial structure and were far away from each other.Conclusions： GST gene is screened from cDNA library of adult Taenia saginata.GST is predicted to be a cytosolic protein and has good application prospect for immunodiagnosis.

关 键 词：牛肉绦虫 谷胱甘肽S-转移酶 基因 蛋白质序列分析 

分 类 号：R383.32[医药卫生—医学寄生虫学]