大孔吸附树脂分离纯化白芍芍药苷的工艺研究  

Technique for Purification of Paeoniflorin from Radix Paeoniae alba with Macroporous Resin

作　　者：樊燕[1] 饶光玲[2] 

机构地区：[1]贵阳护理职业学院,贵州贵阳550003 [2]贵阳医学院药学院,贵州贵阳550004

出　　处：《贵阳医学院学报》2012年第1期30-33,36,共5页Journal of Guiyang Medical College

基　　金：贵州省中药现代化科技产业研究开发项目(合同编号:黔科合中药专字[2003]74)

摘　　要：目的:筛选分离白芍芍药苷的最佳树脂,并对影响分离的各种因素进行系统研究,使分离工艺达到最优化。方法:采用静态与动态的吸附-解吸2种方法,以高效液相色谱(HPLC)法测定芍药苷的含量为评价指标,进行工艺筛选。结果:D101型大孔树脂分离效果最好,其最佳工艺为饱和动态吸附-洗脱量94.5 mg/g(干树脂)、上样液芍药苷质量与树脂质量比1∶10.5、上样液体积与树脂质量比(ml∶g)10∶1,上样液pH值6～7、以2～3 ml/min的速率吸附,用3 BV(树脂床体积)的水、以3～4 ml/min的流速冲洗杂质,用3 BV 40%乙醇、以2～3ml/min的流速洗脱。经D101处理后的芍药苷纯度和回收率均达90%。结论:该方法简单可行,分离效果好,能满足大生产的要求。Objective： To screen the optimal resin for separation of paeoniflorin from Radix paeoniae alba,and to explore various factors affecting separaption,and so as to optimize isolation technique.Methods： Static and dynamic adsorption-desorption methods were adopted to isolate paeoniflorin.Separating efficiency was evaluated by determining paeoniflorin contents in Radix Paeoniae alba with high performance liquid chromatography（HPLC）.Results： Macroporous resin D101 had the best separating efficiency,and the optimum process for purification was as follows： saturated dynamic adsorption-desorption amount was 94.5 mg/g（dry resin）,the ratios of paeoniflorin amount in sample solution to resin amount and the volume of sample solution to resin amount were 1∶10.5 and 10∶1（ml：g） respectively,The pH of sample solution was 6-7,the adsorption-rate was 2-3 ml/min,the impurities were washed with 3 BV（resin bed volume） water and the flow rate was 3-4 ml/min,and 3 BV 40% ethanol was used to elute with a elution rate of 2-3 ml/min.After treated by D101 resin,the purity and recovery of paeoniflorin were 90%.Conclusions： This method is simple,feasible and effective in separation,which can meet industrial requirements.

关 键 词：白芍 色谱法 高压液相 吸附 芍药苷 工艺学 制药 

分 类 号：R948[医药卫生—药剂学]