链脲佐菌素诱导的糖尿病大鼠模型稳定性观察  

The Stability of Type 1 Diabetes Rat Model Induced by STZ and Discussion on Standards of Successful Modeling

作　　者：汪峰[1,2] 杨国珍[1,2] 刘宓[1] 刘莲[1] 罗武[1] 潘卫[1,2] 李兴[1,2] 

机构地区：[1]贵阳医学院医学检验系,贵州贵阳550004 [2]贵阳医学院附院生物化学科,贵州贵阳550004

出　　处：《贵阳医学院学报》2012年第1期40-42,共3页Journal of Guiyang Medical College

基　　金：贵州省科学技术基金项目;黔科合J字[2011]2118号;贵州省教育厅自然科学研究项目;黔教科[2010]032;贵州省教育厅;黔教高发[2006]354号;贵阳医学院研究生教育创新计划专项经费资助项目;合同字第S201101号

摘　　要：目的:观察链脲佐菌素(STZ)诱导的1型糖尿病(T1DM)大鼠模型的稳定性,并探讨有关建模成功的标准。方法:模型组采用STZ按50 mg/kg的剂量进行腹腔注射复制T1DM大鼠模型,对照组SD大鼠则腹腔注射等量的pH4.2、0.1 mol/L柠檬酸-柠檬酸钠缓冲液,分别在腹腔注射后72 h、1周、2周、3周、4周、5周、6周、7周、8周时检测各组大鼠的空腹血糖。结果:参照目前常用的腹腔注射STZ 72 h后空腹血糖值≥16.7 mmol/L即表示建模成功的标准,腹腔注射STZ后72 h、1周、2周、3周、4周、5周、6周、7周、8周的建模成功率分别是100%、80%、75%、70%、70%、70%、70%、70%、70%。结论:如果采用目前常用的建模成功的标准,实际上在T1DM组中有30%的大鼠是未建模成功的,因此建议采用腹腔注射STZ后第3周空腹血糖值≥16.7 mmol/L作为建模成功的标准。Objective： To observe the stability of type 1 diabetes rat model（T1DM） induced by streptozotocin（STZ）,and to explore standards of successful modeling.Methods： T1DM model was established by intraperitoneal injection of STZ with a dose of 50 mg/kg,and rats in control group were given the same volume of pH 4.2,0.1 mol/L citric acid-citric sodium buffer solution instead.The fasting blood glucose of each group was detected respectively in 72 hours,1 week,2 weeks,3 weeks,4 weeks,5 weeks,6 weeks,7 weeks,and 8 weeks after injection of STZ.Results： According to common standards of successful model that the fasting blood glucose greater than or equal to 16.7 mmol/L after intraperitoneal injection of STZ for 72 h,the modeling successful rates of our T1DMs after injection for different time durations were 100%,80%,75%,70%,70%,70%,70%,70% and 70% respectively.Conclusions： Based on the current standards,30% T1DM model rats are failure actually.Fasting blood glucose ≥16.7 mmol/L kept for 3 weeks after injection of STZ as the standard for succesful modeling is proposed here.

关 键 词：链脲佐菌素 糖尿病 胰岛素依赖型 模型 动物 标准 

分 类 号：R587.1[医药卫生—内分泌]