受GA_3诱导的一个水稻伸长相关基因的初步鉴定  

Isolation and Cloning of a Candidate Elongation Related Gene in Rice Induced by GA_3

作　　者：莫肖蓉[1] 董海涛[1] 吴玉良[1] 何祖华[1] 李德葆[1] 

机构地区：[1]浙江大学华家池校区生物技术研究所,浙江杭州310029

出　　处：《中国水稻科学》2000年第1期1-5,共5页Chinese Journal of Rice Science

基　　金：国家自然科学基金! ( 3 940 0 0 80 )

摘　　要：采用 m RNA差异显示技术对受 GA3诱导的水稻伸长相关基因进行了分离和鉴定。经 10 0个引物组合差异显示 ,在获得 2 1个受 GA3诱导表达的 c DNA片段的基础上 ,对其中的 GA15 b片段克隆进行了序列测定。其序列经国际联网BLAST查询表明 GA15 b为伸展蛋白类似蛋白的候选基因片段。定量 PCR进一步确证 GA15 b受 GA3所诱导。Southern杂交表明 GA15Gene expression patterns in rice elongation induced by GA 3 were investigated by using mRNA differential display technique. From 100 combinations of anchor and arbitrary primers, twenty one GA 3 induced cDNA fragments were obtained. One of them was sequenced and shown to encode extensin like protein. Quantitative PCR confirmed that the expression of GA15b was induced by GA 3. Southern blot analysis indicated that the GA15b was a multicopy gene.

关 键 词：水稻 赤霉素 伸展蛋白 基因 差异显示技术 

分 类 号：S511.101[农业科学—作物学]