干旱胁迫下外源水杨酸对黄瓜幼苗膜脂过氧化和光合特性的影响  

Effects of exogenous salicylic acid on membrane lipid peroxidation and photosynthetic characteristics of Cucumis sativus seedlings under drought stress

作　　者：郝敬虹[1] 易旸[1] 尚庆茂[1] 董春娟[1] 张志刚[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所/农业部园艺作物生物学与种质创新重点实验室,北京100081

出　　处：《应用生态学报》2012年第3期717-723,共7页Chinese Journal of Applied Ecology

基　　金：现代农业产业技术体系专项(CARS-25-C-13);国际科技合作项目(2010DFB30550)资助

摘　　要：为了探讨外源水杨酸(SA)提高植物抗旱性的相关机制,研究了干旱胁迫下(基质含水量为饱和持水量的60%和50%),根际施用外源SA对黄瓜幼苗生长、膜脂过氧化、脯氨酸积累、水分利用效率、净光合速率(Pn)和叶绿素荧光参数的影响.结果表明:SA处理能够缓解干旱胁迫对黄瓜幼苗生长、Pn和水分利用效率的抑制,减小膜脂过氧化程度,促进脯氨酸的积累;添加外源SA显著减小了干旱胁迫下黄瓜幼苗的PSⅡ最大光化学效率、PSⅡ实际光化学效率、PSⅡ潜在活性、PSⅡ有效光化学效率和光化学猝灭系数的下降幅度,抑制了非光化学猝灭系数的升高.添加外源SA可以缓解干旱胁迫造成的膜脂过氧化对膜系统的氧化损伤,并通过增强PSⅡ反应中心活性提高了Pn,有助于水分的利用,同时增大渗透调节能力,减少水分的散失,提高水分利用效率,从而增强植株对干旱的适应能力.To approach the related mechanisms of exogenous salicylic acid（SA） in improving plant drought-resistance,this paper studied the effects of applying exogenous SA to the rhizosphere on the plant growth,membrane lipid peroxidation,proline accumulation,water use efficiency,net photosynthetic rate（Pn）,and chlorophyll fluorescence parameters of cucumber（Cucumis sativus） seedlings under drought stresses（60% and 50% of saturated water capacity）.Applying SA relieved the inhibitory effects of drought stress on plant growth,Pn,and water use efficiency,decreased membrane lipid peroxidation,and promoted proline accumulation.Meanwhile,the SA decreased the decrements of the maximum photochemical efficiency of PSⅡ,actual photochemical efficiency of PSⅡ,potential activity of PSⅡ,effective photochemical efficiency of PSⅡ,and photochemical quenching coefficient under drought stress significantly,and limited the increase of non-photochemical quenching coefficient.All the results suggested that applying exogenous SA could alleviate the oxidation damage of cell membrane resulted from the drought-caused membrane lipid peroxidation,improve the Pn by increasing PSⅡ activity to benefit water utilization,enhance the regulation capability of osmosis to decrease water loss and increase water use efficiency,and thereby,improve the plant drought-resistance.

关 键 词：水杨酸 干旱胁迫 黄瓜 膜脂过氧化 水分利用效率 叶绿素荧光 

分 类 号：S642.2[农业科学—蔬菜学]