低强度脉冲超声波联合牙周组织翻瓣移植修复Beagle犬骨上缺损型牙周病  

Low-intensity pulsed ultrasound combined with periodontal flap surgery for the repair of bone-defect periodontitis in Beagle dogs

作　　者：李娜[1] 卢礼 宋锦璘[1] 邓锋[1] 赵纯亮 王智彪 

机构地区：[1]重庆医科大学附属口腔医院冉家坝院区正畸科,重庆市401147 [2]解放军第三军医大学第二附属医院口腔科,重庆市400037 [3]超声医学工程重庆市市级重点实验室,重庆市400016

出　　处：《中国组织工程研究与临床康复》2012年第5期766-770,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金(30870754);重庆市发展和改革委员会高技术产业重大产业技术开发项目(渝发改技[2007]1110号);重庆卫生局科研项目(2008-2-254)资助~~

摘　　要：背景:近年来,低强度脉冲超声波联合牙周翻瓣对骨上缺损型牙周炎组织的修复效应的尚未见报道。目的:观察低强度脉冲超声波联合牙周翻瓣术对Beagle犬骨上缺损型牙周炎组织的修复效应。方法:在4只Beagle犬左下颌第二、三、四前磨牙处构建骨上缺损型牙周炎模型,经改良Widman翻瓣及根面处理后随机分为对照组(0mW/cm2)和ISATA30mW/cm2×20min/d、ISATA60mW/cm2×20min/d低强度脉冲超声波处理组。结果与结论:低强度脉冲超声波处理前后各组牙龈组织温度变化值差异无显著性意义(P>0.05);6周后对照组和低强度脉冲超声波处理组,以及处理组之间在牙周临床指标上差异无显著性意义(P>0.05);组织学切片苏木精-伊红染色显示低强度脉冲超声波处理组成骨细胞增生及骨陷窝较对照组明显,尤其是ISATA30mW/cm2×20min/d低强度脉冲超声波处理组;Masson染色显示低强度脉冲超声波处理组红染较对照组明显;低强度脉冲超声波处理组和对照组均形成长结合上皮。提示低强度脉冲超声波具有潜在的促骨上缺损型牙周炎组织修复作用。BACKGROUND:In recent years,there are no reports regarding tissue repair effects of low-intensity pulsed ultrasound(LIPUS) combined with periodontal flap surgery treatment on bone-defect periodontitis.OBJECTIVE:To observe the effects of LIPUS combined with periodontal flap surgery on the repair of bone-defect periodontitis in Beagle dogs.METHODS:Bone-defect periodontitis models were established in the left mandibular second,third and fourth premolars of four Beagle dogs.The models were randomly divided into a control group(0 mW/cm2) and two LIPUS treatment groups(ISATA 30 mW/cm2×20 min/d,ISATA 60 mW/cm2×20 min/d) after modified Widman flap surgery and root treatment.RESULTS AND CONCLUSION:There was no significant difference in the change of gingival tissue's surface temperature before and after LIPUS irradiation(P > 0.05).There was no significant difference of periodontal clinical parameters in the LIPUS and control groups,as well as between the two LIPUS groups after 6 weeks treatment(P > 0.05).Histological analysis(hematoxylin-eosin staining) indicated that osteoblast proliferation and bone lacunae in the LIPUS groups,especially in ISATA 30 mW/cm2× 20 min/d group,were more mature than those in the control group.Masson staining showed red staining was more obvious in the LIPUS groups than in the control group.Long-junction epithelium was found in all the three groups.LIPUS may have some potential reparative effects on the promotion of alveolar bone maturation.

关 键 词：低强度脉冲超声波 牙周翻瓣术 BEAGLE犬 骨缺损 牙周炎组织 

分 类 号：R617[医药卫生—外科学]