青蒿琥酯对迟发型超敏反应小鼠脾脏T淋巴细胞的免疫调节作用  

Immunomodulatory activity of artesunate to T lymphocytes from the spleens of delayed-type hypersensitivity mice

作　　者：李覃[1,2] 陈虹[2,3] 白淑芳[3] 韦娜[3] 张实[3] 

机构地区：[1]武警医学院免疫学教研室,天津市300162 [2]武警医学院天津市职业与环境危害生物标志物重点实验室,天津市300162 [3]武警医学院生药学教研室,天津市300162

出　　处：《中国组织工程研究与临床康复》2012年第5期843-846,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：中国博士后科学基金面上项目(20090461498);天津市应用基础与前沿技术研究计划(11JCYBJC14600);中国博士后科学基金特别资助项目(201104796)~~

摘　　要：背景:青蒿琥酯是青蒿素低毒、高效的衍生物,具有免疫调节功能,但其具体机制仍需研究阐明。目的:初步探讨青蒿琥酯对迟发型超敏反应小鼠脾脏T淋巴细胞的免疫调节作用。方法:建立迟发型超敏反应小鼠模型,40只小鼠随机数字表法均分正常对照组、青蒿琥酯干预组、p-38 MAPK抑制剂组、基质对照组进行实验观察。T淋巴细胞转化实验检测小鼠T淋巴细胞增殖水平;Western blot方法检测p38丝裂酶原激活蛋白激酶(p38 MAPK)蛋白的活性表达。结果与结论:局部给药后青蒿琥酯明显减轻迟发型超敏反应小鼠耳肿胀、降低脾指数、抑制刀豆蛋白A诱导的T淋巴细胞增殖;同时减弱p38MAPK的磷酸化活性表达。提示青蒿琥酯可以有效抑制小鼠迟发型超敏反应,其作用途径可能与抑制p38MAPK信号通路有关。BACKGROUND：Artesunate is a derivant of high efficiency and low toxicity from artemisinin,which has been shown to affect immune response.However,the underlying mechanism remains elusive.OBJECTIVE：To investigate the immunoregulatory mechanism of artesunate to T lymphocytes from the spleens of delayed-type hypersensitivity mice.METHODS：The effects of artesunate on the ear swelling and spleen index were measured in delayed-type hypersensitivity mice.The transformation of spleen lymphocytes was used to detect the proliferation of T cells.Western blot assay was applied to investigate the p38 mitogen-activated protein kinase（MAPK） activation.The proliferation of T lymphocytes was detected by T lymphocyte transformation test.MAPK activity was detected by western blot method.RESULTS AND CONCLUSION：Topical application of artesunate could suppress the increase in ear thickness,spleen index and ConA-induced T lymphocytes proliferation significantly.Furthermore,artesunate was observed to decrease the phosphorylation of p38 MAPK.These demonstrated that artesunate had an anti-delayed-type hypersensitivity effect through modulating p38MAPK pathways,which might be beneficial for immune-related diseases.

关 键 词：青蒿琥酯 免疫调节 迟发型超敏反应 脾脏 T淋巴细胞 P38 MAPK 

分 类 号：R318[医药卫生—生物医学工程]