牡丹组织培养若干影响因子研究  

Several factors influencing tissue culture of Paeonia suffruticosa

作　　者：殷丽青[1,2] 王新其[1] 胡永红[3,2] 刘炤[3,2] 李秀芬 张建军[1] 

机构地区：[1]上海市农业科学院林木果树研究所,上海201403 [2]上海城市植物资源开发应用工程技术研究中心,上海200231 [3]上海植物园,上海200231

出　　处：《上海农业学报》2012年第1期17-21,共5页Acta Agriculturae Shanghai

基　　金：上海市科技兴农重点攻关项目(沪农科攻字2008第10-4号);上海市重点科技攻关项目(10391901200;10dz2253700)资助

摘　　要：以‘太平红'牡丹为材料,研究了不同灭菌方式、抗褐化物质、激素配比、培养基、生根培养条件对牡丹组织培养的影响。结果表明:以0.1%升汞液消毒8 min,再用7%次氯酸钠溶液消毒8 min灭菌效果最佳,鳞芽、土芽和种子的污染率分别为0、4.5%和22.0%;在改良MS培养基中联合添加PVPP 0.5 g/L和AC 0.5 g/L可有效减少牡丹外植体的褐化;改良MS+BA 1.0 mg/L+NAA 0.2 mg/L+GA 0.3 mg/L+CH 0.5 g/L+PVPP0.5 g/L+AC 0.5 g/L适宜牡丹鳞芽的诱导;MS+BA 1.5 mg/L+NAA 0.2 mg/L+PVPP 1.0g/L适宜于牡丹芽的增殖;1/2MS+IBA 5 mg/L+AC 0.5 g/L适用于牡丹试管苗生根。The peony cultivar ＇Taipinghong was used as a test material,and tissue culture of peony was studied under using different sterilization methods, anti-browning materials, hormone recipes, media and rooting conditions. The result showed that the effect of sterilizing with 0. 1% mercuric chloride solution for 8 min and then with 7% sodium hypochlorite solution for 8 min was the best,in which the contamination rate was zero for the scaly buds,4.5% for the underground buds and 22.0% for the seeds;Adding 0.5 g/L PVPP and 0.5 g/L AC in modified MS medium could effectively mitigate browning of the peony explants; Modified MS ＋ 1.0 mg/L BA ＋ 0.2 mg/L NAA ＋ 0.3 mg/L GA ＋ 0.5 g/L CH ＋ 0.5 g/L PVPP ＋ 0.5 g/L AC was suitable for the scaly bud induction; MS ＋ 1.5 mg/L BA ＋ 0.2 mgJL NAA ＋ 1.0 g/L PVPP was suitable for the peony bud propagation;And 1[2 MS ＋ 5.0 mg/L IBA ＋ 0.5 g/L AC was suitable for the test-tube seedling rooting.

关 键 词：牡丹 组织培养 增殖 褐化 生根 

分 类 号：S685.11[农业科学—观赏园艺]