铁皮石斛种质资源研究中的DNA条形码初探  

Preliminary study on the DNA barcoding in Dendrobium officinale germplasm resources

作　　者：姚领爱[1] 胡之璧[2] 郑志仁[3] 周吉燕[2] 王莉莉[2] 化海霞[2] 黎万奎[2] 

机构地区：[1]上海中医药大学基础医学院,上海201203 [2]上海中医药大学中药研究所,上海201203 [3]上海师范大学生命与环境科学学院,上海200234

出　　处：《上海农业学报》2012年第1期49-54,共6页Acta Agriculturae Shanghai

基　　金：国家自然科学基金项目(30873386);国家自然科学基金项目(81130070);上海市复方中药重点实验室(上海中医药大学)开放课题基金项目(11DZ2270900);上海市教委重点学科第5期<中西医结合基础>(J50301)资助

摘　　要：为了规范铁皮石斛种质资源及相关产品鉴定,开展条形码基因的初步筛选,通过对铁皮石斛叶绿体相关基因(rbcL、trnH-psbA、rbl16)进行扩增、测序、序列对比、聚类分析发现:rbcL基因可将市场上常易混淆的石斛种苗有效区分,但rbcL基因作为石斛属条形码基因需要扩大范围进一步深入研究。In order to regulate the identification of Dendrobium officinale germplasm resources and relative products, preliminary screening of the barcoding genes was conducted by amplification, sequencing,sequence alignment and cluster analysis of D. officinale chloroplast-related genes （rbcL, trnH-psbA, rb116）. The result showed that the dendrobium seedlings easy to be confused in the market could effectively be distinguished by the rbcL gene, but further research should be made before the rbcL ene could be used as a barcoding gene of Dendrobium.

关 键 词：铁皮石斛 DNA条形码 种质资源 

分 类 号：S682.31[农业科学—观赏园艺]