利用F_2群体对深水稻抗纹枯病QTLs定位  

QTL mapping for sheath blight resistance of deep water rice by using F_2 population

作　　者：付冬[1,2] 陈亮[1] 刘毅[1] 楼巧君[1] 李明寿[1] 熊良[1] 罗利军[1,2] 

机构地区：[1]上海市农业生物基因中心,上海201106 [2]华中农业大学植物科技学院,武汉430070

出　　处：《上海农业学报》2012年第1期55-59,共5页Acta Agriculturae Shanghai

基　　金：国家重点基础研究发展计划(2010CB125901);农业部转基因重大专项(2008ZX08001-002);上海市自然科学基金项目(09ZR1428200)资助

摘　　要：构建了一个由‘HH1B'和‘RSB03'杂交、包含286个个体的F_2群体,对群体及亲本人工接种纹枯病菌,综合应用病级(DR)、病斑长度(LL)、病斑高度(LH)及相对病斑长度(RLL)等指标进行抗性评价。通过QTLs定位分析,检测到4个与纹枯病抗性相关的QTLs位点,分别分布于第4、11及12染色体上,其中第11染色体上的位点同时控制DR与RLL,另外检测到7个与抽穗期(HD)及株高(PH)相关的QTLs位点。An F2 population consisting of 286 individuals was constructed by crossing between ＇HHIB and ＇RSB03 ,a deep water rice variety with good resistance to sheath blight. The population and parents were artificially inoculated with sheath blight fungus and their resistances to sheath blight were evaluated in terms of such indexes as disease rating（DR）,lesion length（LL）,lesion height（LH） and relative lesion length（RLL,or the ratio of lesion length to plant height）. It was found by QTL mapping that four QTLs relative to sheath blight resistance were distributed on the 4th, l lth and 12th chromosomes, and the QTL on the 11 th chromosome simultaneously controlled the DR and RLL. Also a total of 7 QTLs relative to heading date（HD）and plant height（PH）were detected.

关 键 词：深水稻 纹枯病 F_2群体 数量性状座位 

分 类 号：S511[农业科学—作物学] Q78[生物学—分子生物学]